LMPD Database

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LMP007603

UniProt Annotations

Entry Information
Gene Nameenoyl-CoA hydratase/epimerase and isomerase/3-hydroxyacyl-CoA dehydrogenase
Protein EntryFADJ_ECOLI
UniProt IDP77399
SpeciesE. coli
Comments
Comment typeDescription
Catalytic Activity(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl- CoA + H(2)O.
Catalytic Activity(S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH.
Catalytic Activity(S)-3-hydroxybutanoyl-CoA = (R)-3- hydroxybutanoyl-CoA.
FunctionCatalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities. Strongly involved in the anaerobic degradation of long and medium-chain fatty acids in the presence of nitrate and weakly involved in the aerobic degradation of long-chain fatty acids. {ECO:0000269|PubMed:12270828, ECO:0000269|PubMed:12535077}.
InductionUnlike the aerobic pathway, the anaerobic pathway is not strongly repressed by FadR regulatory protein.
PathwayLipid metabolism; fatty acid beta-oxidation.
SimilarityIn the central section; belongs to the 3-hydroxyacyl- CoA dehydrogenase family. {ECO:0000305}.
SimilarityIn the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family. {ECO:0000305}.
Subcellular LocationCytoplasm {ECO:0000250}.
SubunitHeterotetramer of two alpha chains (FadJ) and two beta chains (FadI). {ECO:0000250}.