LMPD Database

LMP010021

UniProt Annotations

Entry Information
Gene Nameceramide kinase
Protein EntryCERK_ARATH
UniProt IDQ6USK2
SpeciesArabidopsis
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=24.4 uM for C8 ceramide {ECO:0000269|PubMed:14563678}; pH dependence: Optimum pH is 8.2 (at 30 degrees Celsius). {ECO:0000269|PubMed:14563678};
Catalytic ActivityATP + ceramide = ADP + ceramide 1-phosphate. {ECO:0000269|PubMed:14563678}.
CofactorName=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000269|PubMed:14563678};
Disruption PhenotypeEnhanced apoptotic-like cell death late in development. {ECO:0000269|PubMed:14563678}.
FunctionCatalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. Possesses high activity on ceramide analogs (C6, C8 synthetic ceramides) and lower activity on C6 and C8 dihydroceramides. Has weak activity on natural ceramides (a mixture of ceramides from bovine brain) and the synthetic substrate C2 ceramide. Has very poor activity on diacylglycerol and sphingosine. Ceramide is a critical sphingolipid metabolite that induces programmed cell death (PCD) in plants and ceramide-1- phosphate has a PCD suppressive effect. Thus, ceramide phosphorylation plays a role in the modulation of PCD and CERK activity is crucial for the maintenance of cell viability. {ECO:0000269|PubMed:14563678}.
InductionBy infection with bacterial pathogen P.syringae. {ECO:0000269|PubMed:14563678}.
Sequence CautionSequence=BAA97392.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
SimilarityContains 1 DAGKc domain. {ECO:0000255|PROSITE- ProRule:PRU00783}.