LMPD Database

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LMP010561

UniProt Annotations

Entry Information
Gene Namelong chain acyl-CoA synthetase 1
Protein EntryLACS1_ARATH
UniProt IDO22898
SpeciesArabidopsis
Comments
Comment typeDescription
Catalytic ActivityATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA. {ECO:0000269|PubMed:12177484}.
CofactorName=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
Disruption PhenotypeIn stem, fewer and flatter wax crystals and disorganized cuticle proper and thinner cuticular layer. Reduced amount of wax in all chemical classes on the stem and leaf, except in the very long-chain fatty acid (VLCFA) class with acids longer than 24 carbons (C(24)). {ECO:0000269|PubMed:19392700, ECO:0000269|PubMed:20237894}.
FunctionActivation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Acts in both the wax and cutin pathways. Preferentially uses palmitate, palmitoleate, linoleate and eicosenoate. Seems to have a specific activity against very long-chain fatty acid (VLCFA) class with acids longer than 24 carbons (C(24)). {ECO:0000269|PubMed:12228482, ECO:0000269|PubMed:19392700, ECO:0000269|PubMed:20237894}.
PathwayLipid metabolism; fatty acid metabolism.
SimilarityBelongs to the ATP-dependent AMP-binding enzyme family. {ECO:0000305}.
Subcellular LocationEndoplasmic reticulum {ECO:0000269|PubMed:20237894}.
Tissue SpecificityEpidermal-specific expression along the entire stem. In cauline leaves, was expressed over the entire leaf surface, most strongly in trichomes and guard cells, but not in mesophyll cells. In flowers, the expression was detected in the stigma and filaments of the stamens, and in the carpel was expressed specifically in ovaries. In roots, was expressed in primary and lateral roots, but not in the root tips. {ECO:0000269|PubMed:19392700, ECO:0000269|PubMed:20237894}.