LMPD Database

New search | Record Overview | Ontology/Pathway Information | Domain Information | UniProt Annotations | Related Proteins
LMP010562

UniProt Annotations

Entry Information
Gene Namelong-chain acyl-CoA synthetase 2
Protein EntryLACS2_ARATH
UniProt IDQ9XIA9
SpeciesArabidopsis
Comments
Comment typeDescription
Catalytic ActivityATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA. {ECO:0000269|PubMed:12177484}.
CofactorName=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
Disruption PhenotypeDwarf phenotype with smaller, wrinkled leaves and overall reduced vigor. Higher water loss rate and susceptibility to drought stress. Defective in the cuticular membrane. Strong resistance to virulent Botrytis cinerea and enhanced susceptibility to avirulent Pseudomonas syringae. {ECO:0000269|PubMed:14973169, ECO:0000269|PubMed:17396154, ECO:0000269|PubMed:17434992, ECO:0000269|PubMed:20237894}.
FunctionActivation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Acts in the cutin pathway. Preferentially uses palmitate, palmitoleate, oleate and linoleate. Required for repression of lateral root formation through its role in cutin biosynthesis and subsequent aerial tissues permeability. {ECO:0000269|PubMed:14973169, ECO:0000269|PubMed:15960613, ECO:0000269|PubMed:17396154, ECO:0000269|PubMed:17434992, ECO:0000269|PubMed:18952782}.
InductionPositively regulated by WIN1. {ECO:0000269|PubMed:17449808}.
PathwayLipid metabolism; fatty acid metabolism.
SimilarityBelongs to the ATP-dependent AMP-binding enzyme family. {ECO:0000305}.
Subcellular LocationEndoplasmic reticulum {ECO:0000269|PubMed:20237894}.
Tissue SpecificityExpressed along the entire length of the stem, but expression was not entirely epidermal specific, with some expression found in internal cell layers as well. Was expressed in leave epidermal cells, flowers (sepals, petals, stamens, filaments and carpel), siliques and developing seeds. In roots, expression was detected in an internal cell layer, probably the endodermal layer. {ECO:0000269|PubMed:14973169, ECO:0000269|PubMed:19392700}.