LMPD Database

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LMP010758

UniProt Annotations

Entry Information
Gene Namefatty acid beta-oxidation multifunctional protein MFP2
Protein EntryMFP2_ARATH
UniProt IDQ9ZPI5
SpeciesArabidopsis
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=240 uM for crotonyl-CoA {ECO:0000269|PubMed:10521521};
Catalytic Activity(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl- CoA + H(2)O.
Catalytic Activity(3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl- CoA.
Catalytic Activity(S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH.
Catalytic Activity(S)-3-hydroxybutanoyl-CoA = (R)-3- hydroxybutanoyl-CoA.
Developmental StageExpression increases rapidly during seed germination to reach a peak at 2-3 days after imbibition (DAI) and then declines to basal levels at 5 DAI. {ECO:0000269|PubMed:10816107}.
Disruption PhenotypeAccumulation of long-chain acyl-CoA substrates and increased size of peroxisomes. {ECO:0000269|PubMed:16507084}.
DomainThe epimerase and isomerase activities are contained in the N-terminal region while the dehydrogenase activity is in the C-terminal region. {ECO:0000250}.
FunctionInvolved in peroxisomal fatty acid beta-oxidation during seed germination. Possesses enoyl-CoA hydratase activity against long chain substrates (C14-C18) and 3-hydroxyacyl-CoA dehydrogenase activity against chains of variable sizes (C6-C18). {ECO:0000269|PubMed:10521521, ECO:0000269|PubMed:16507084, ECO:0000269|PubMed:20463021}.
PathwayLipid metabolism; fatty acid beta-oxidation.
SimilarityIn the central section; belongs to the 3-hydroxyacyl- CoA dehydrogenase family. {ECO:0000305}.
SimilarityIn the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family. {ECO:0000305}.
Subcellular LocationGlyoxysome {ECO:0000305}. Peroxisome {ECO:0000305}.
Tissue SpecificityHighly expressed in senescing leaves and at lower levels in flowers and siliques. {ECO:0000269|PubMed:10816107}.