LMPD Database

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LMP011298

UniProt Annotations

Entry Information
Gene Namesphingoid base hydroxylase 1
Protein EntrySBH1_ARATH
UniProt IDQ8VYI1
SpeciesArabidopsis
Comments
Comment typeDescription
Catalytic ActivitySphinganine + NADPH + O(2) = phytosphingosine + NADP(+) + H(2)O. {ECO:0000269|PubMed:11297741}.
CofactorName=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000250};
Disruption PhenotypeNo visible phenotype; due to the redundancy with SBH2. Sbh1 and sbh2 double mutants are severely dwarfed, do not progress from vegetative to reproductive growth and have enhanced expression of programmed cell death associated-genes. {ECO:0000269|PubMed:18612100}.
DomainThe histidine box domains may contain the active site and/or be involved in metal ion binding.
FunctionInvolved in sphingolipid trihydroxy long-chain base (4- hydroxysphinganine) biosynthesis. Can use C18- and C20-sphinganine as substrates to produce C18- and C20-phytosphinganines (D-ribo-2- amino-1,3,4-trihydroxyoctadecane and -eicosane). {ECO:0000269|PubMed:11297741, ECO:0000269|PubMed:18612100}.
PathwayMembrane lipid metabolism; sphingolipid biosynthesis.
Sequence CautionSequence=AAG12703.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=AAG52550.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};
SimilarityBelongs to the sterol desaturase family. {ECO:0000305}.
Subcellular LocationEndoplasmic reticulum membrane {ECO:0000269|PubMed:18612100}; Multi-pass membrane protein {ECO:0000269|PubMed:18612100}.
Tissue SpecificityUbiquitous, with higher levels in flowers and roots. {ECO:0000269|PubMed:18612100}.