LMPD Database

LMP012494

UniProt Annotations

Entry Information
Gene Nameacyl-CoA synthetase long-chain family member 1
Protein EntryACSL1_RAT
UniProt IDP18163
SpeciesRat
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=649 uM for ATP ; KM=6.4 uM for CoA ; KM=5.0 uM for palmitate ; Vmax=1695 nmol/min/mg enzyme with palmitate as substrate ;
Catalytic ActivityATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.
CofactorName=Mg(2+); Xref=ChEBI:CHEBI:18420;
Developmental StageLevels remain constant during development.
FunctionActivation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses oleate, arachidonate, eicosapentaenoate and docosahexaenoate as substrates.
InductionBy high fat and high carbohydrate diets.
Miscellaneous5 rat isozymes encoded by different genes have been described. ACSL6 corresponds to isozyme 2 (ACS2)
SimilarityBelongs to the ATP-dependent AMP-binding enzyme family
Subcellular LocationMitochondrion outer membrane ; Single-pass type III membrane protein . Peroxisome membrane ; Single-pass type III membrane protein {ECO:0000250}. Microsome membrane ; Single-pass type III membrane protein . Endoplasmic reticulum membrane ; Single-pass type III membrane protein .
Tissue SpecificityLiver, heart, epididymal adipose and to a lesser extent brain, small intestine and lung.