LMPD Database

LMP012848

UniProt Annotations

Entry Information
Gene Nameacyl-CoA synthetase long-chain family member 4
Protein EntryACSL4_RAT
UniProt IDO35547
SpeciesRat
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=34 uM for ATP ; KM=4.1 uM for CoA ; KM=5.4 uM for palmitate ; KM=19.5 uM for oleate ; KM=10 uM for arachidonate ; Vmax=2800 nmol/min/mg enzyme with palmitate as substrate ; Vmax=673 nmol/min/mg enzyme with oleate as substrate ; Vmax=4200 nmol/min/mg enzyme with arachidonate as substrate ;
Catalytic ActivityATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.
CofactorName=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence= ;
FunctionActivation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses arachidonate and eicosapentaenoate as substrates.
Miscellaneous5 rat isozymes encoded by different genes have been described. ACSL6 corresponds to isozyme 2 (ACS2)
SimilarityBelongs to the ATP-dependent AMP-binding enzyme family