LMPD Database

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LMP012851

UniProt Annotations

Entry Information
Gene Nameacyl-CoA synthetase long-chain family member 3
Protein EntryACSL3_RAT
UniProt IDQ63151
SpeciesRat
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative initiation; Named isoforms=2; Name=Long; IsoId=Q63151-1; Sequence=Displayed; Name=Short; IsoId=Q63151-2; Sequence=VSP_018649;
Biophysicochemical PropertiesKinetic parameters: KM=402 uM for ATP ; KM=3.0 uM for CoA ; KM=4.3 uM for palmitate ; KM=5.1 uM for oleate ; KM=8.9 uM for arachidonate ; Vmax=2306 nmol/min/mg enzyme with palmitate as substrate ; Vmax=2399 nmol/min/mg enzyme with oleate as substrate ; Vmax=4022 nmol/min/mg enzyme with arachidonate as substrate ;
Catalytic ActivityATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.
CofactorName=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence= ;
Developmental StageDetected 5 days after birth, increased to a maximal level at 15 days, and then decreased gradually to 10% of its maximum level in adult.
FunctionAcyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 is required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins) (By similarity). Has mainly an anabolic role in energy metabolism. Mediates hepatic lipogenesis. Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates. Both isoforms exhibit the same level of activity. {ECO:0000250, ECO:0000269|PubMed:19737935}.
Miscellaneous5 rat isozymes encoded by different genes have been described. ACSL6 corresponds to isozyme 2 (ACS2)
SimilarityBelongs to the ATP-dependent AMP-binding enzyme family
Subcellular LocationMitochondrion outer membrane ; Single-pass type III membrane protein . Peroxisome membrane ; Single-pass type III membrane protein {ECO:0000250}. Microsome membrane ; Single-pass type III membrane protein . Endoplasmic reticulum membrane ; Single-pass type III membrane protein .
Tissue SpecificityPredominantly expressed in the brain, and to a much lesser extent, in lung, adrenal gland, kidney, small intestine, and adipose tissue but not detected in heart or liver.