Microarray analysis of BMDM cells treated with 25-OH-cholesterol
(Normal media (M2) or lipid-depleted media+compactin(M3))
(Full timecourse)

Gene symbol: Entrez Gene ID: Description:
M2 2H RATIO: M2 8H RATIO: M2 24H RATIO:
M3 2H RATIO: M3 8H RATIO: M3 24H RATIO:
Records per page: Sort by: Group by Gene:

Introduction

Bone marrow-derived macrophages were obtained from tibias and femurs of mice as per protocol PP0000004702 (Harvesting and Plating Thioglycollate Elicited Macrophages for LM Distribution, pdf). Cells treatments consisted of a) Control (no treatment); b) treatment with 25-OH-cholesterol at time 0; c) lipid-depleted media containing compactin; d) treatment with 25-OH-cholesterol at time 0 in lipid-depleted media containing compactin.

Data normalization

Data normalization was performed as described in J Mol Endocrinol Sasik et al. 33 (1): 1 (PDF)

Array design

45,214 spots on the array
41,034 data spots
38,838 unique probes (6428 probes have no gene id)
21,609 unique mouse genes

Each gene was represented by 1 - 9 probes
Each probe was spotted 1 or 10 time

logo LIPID MAPS is funded by a Wellcome Trust.