The National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) in collaboration with the National Institute of Standards (NIST) recently produced a human plasma standard reference material (SRM 1950) for metabolite analysis. The SRM was prepared by obtaining plasma samples from 100 individuals between 40 and 50 years of age, whose ethnicity was representative of the US population and that included an equal number of men and women.
The intent of the NIDDK/NIST project was to provide a reference material that would be publically available to researchers and that could be used by the clinical chemistry community to identify plasma metabolites for diagnostic purposes. Signature metabolites could then be further probed for their usefulness as disease biomarkers.
The LIPID MAPS Consortium has undertaken the task to analyze this SRM by systematically identifying and quantifying the lipid molecular species in the six main categories of mammalian lipids. The quantitative levels of over 500 different lipids present in this reference human plasma sample are presented here.
Lipidomics reveals a remarkable diversity of lipids in human plasma.
Quehenberger O, Armando AM, Brown AH, Milne SB, Myers DS, Merrill AH, Bandyopadhyay S, Jones KN, Kelly S, Shaner RL, Sullards CM, Wang E, Murphy RC, Barkley RM, Leiker TJ, Raetz CR, Guan Z, Laird GM, Six DA, Russell DW, McDonald JG, Subramaniam S, Fahy E, Dennis EA.
J Lipid Res , [Epub ahead of print] (2010)