Compactin is a potent competitive inhibitor of hydroxymethylglutaryl coenzyme A reductase,
the rate-limiting enzyme in cholesterol biosynthesis.
Experiments were conducted with RAW264.7 cells fed 10% fetal calf serum, as per protocol PP0000002800 (pdf).
3-timepoint study: Measurements were taken at 0, 12, and 24hrs for: (i) compactin, (ii) Kdo2-Lipid A, (iii) compactin + Kdo2-Lipid A. and (iv) control.
8-timepoint study: Measurements were taken at 0, 0.5,1,2,4,8, 12, and 24hrs for: (i) compactin, (ii) Kdo2-Lipid A, (iii) compactin + Kdo2-Lipid A. and (iv) control.
A Mouse Macrophage Lipidome
Dennis EA, Deems RA, Harkewicz R, Quehenberger O, Brown HA, Milne SB, Myers DS, Glass CK, Hardiman GT, Reichart D, Merrill AH, Sullards MC, Wang E, Murphy RC, Raetz CR, Garrett T, Guan Z, Ryan AC, Russell DW, McDonald JG, Thompson BM, Shaw WA, Sud M, Zhao Y, Gupta S, Maurya MR, Fahy E, Subramaniam S. J Biol Chem
(2010) [Epub ahead of print]
Kdo2-Lipid A of Escherichia coli, a defined endotoxin that activates macrophages via TLR-4.
Raetz C, Garrett T, Reynolds C, Shaw W, Moore J, Smith JD, Ribeiro A, Murphy R, Ulevitch R, Fearns C, Reichart D, Glass C, Benner C, Subramaniam S, Harkewicz R, Bowers-Gentry R, Buczynski M, Cooper J, Deems R and Dennis E. Journal of Lipid Research 47, 1097-1111 (2006)