Lipidomics studies on macrophages - RAW 264.7 cells treated with Kdo2-Lipid A and compactin

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Introduction

Compactin is a potent competitive inhibitor of hydroxymethylglutaryl coenzyme A reductase, the rate-limiting enzyme in cholesterol biosynthesis.
Experiments were conducted with RAW264.7 cells fed 10% fetal calf serum, as per protocol PP0000002800 (pdf).
3-timepoint study: Measurements were taken at 0, 12, and 24hrs for: (i) compactin, (ii) Kdo2-Lipid A, (iii) compactin + Kdo2-Lipid A. and (iv) control.
8-timepoint study: Measurements were taken at 0, 0.5,1,2,4,8, 12, and 24hrs for: (i) compactin, (ii) Kdo2-Lipid A, (iii) compactin + Kdo2-Lipid A. and (iv) control.

References

A Mouse Macrophage Lipidome Dennis EA, Deems RA, Harkewicz R, Quehenberger O, Brown HA, Milne SB, Myers DS, Glass CK, Hardiman GT, Reichart D, Merrill AH, Sullards MC, Wang E, Murphy RC, Raetz CR, Garrett T, Guan Z, Ryan AC, Russell DW, McDonald JG, Thompson BM, Shaw WA, Sud M, Zhao Y, Gupta S, Maurya MR, Fahy E, Subramaniam S. J Biol Chem (2010) [Epub ahead of print]
PMID:20923771

Kdo2-Lipid A of Escherichia coli, a defined endotoxin that activates macrophages via TLR-4. Raetz C, Garrett T, Reynolds C, Shaw W, Moore J, Smith JD, Ribeiro A, Murphy R, Ulevitch R, Fearns C, Reichart D, Glass C, Benner C, Subramaniam S, Harkewicz R, Bowers-Gentry R, Buczynski M, Cooper J, Deems R and Dennis E. Journal of Lipid Research 47, 1097-1111 (2006)
PMID:16479018 [doi:10.1194/jlr.M600027-JLR200]

logo LIPID MAPS is funded by a Wellcome Trust.