Protocols

Procedure Protocols

General

Thawing and Passage ProcedurePP0000000101 
Thawing and passage procedure for RAW 264.7 cellsPP0000000100Added 08/27/07
Use of AnimalsPP0000001100Added 08/27/07
Ordering MicePP0000001200Added 08/27/07
Ordering Mice for Foam CellsPP0000002900Added 08/22/06
LIPID MAPS DNA AssayPP0000002700 
Fatty Acid Mass Spectrometry ProtocolPP0000003800Added 10/09/12
Fatty Acid Mass Spectrometry ProtocolPP0000005300Added 10/09/12
Fatty Acid Mass Spectrometry ProtocolPP0000005301Added 10/09/12
Harvesting and Plating Thioglycollate Elicited Macrophages for LM DistributionPP0000004101Added 05/21/08
Core D - Procedure Protocol ATP, Kdo2-Lipid A TreatmentPP0000004601Added 01/04/10
Core D - Procedure Protocol ATP, Kdo2-Lipid A TreatmentPP0000004602Added 01/12/10
Procedure Protocol for Harvesting and Plating Bone Marrow Derived Macrophages for LIPID MAPS DistributionPP0000004701Added 01/04/10
Procedure Protocol for Harvesting and Plating Bone Marrow Derived Macrophages for LIPID MAPS DistributionPP0000004702Added 01/12/10
Protocol ATP/POVPC-peptide Costimulation Time coursePP0000005101Added 10/09/12
Protocol ATP/PAPC/oxidized PAPC Costimulation Time coursePP0000005200Added 10/09/12

Kdo2-Lipid A Treatment

LPS Induction (Serum-deprived)PP0000000600 
10% Serum Kdo2-Lipid A TreatmentPP0000001000Added 08/27/07
10% Serum Kdo2-Lipid A TreatmentPP0000001001 
10% Serum Kdo2-Lipid A TreatmentPP0000001002 
10% Serum Kdo2-Lipid A TreatmentPP0000001003 
10% Serum Kdo2-Lipid A TreatmentPP0000001004 
0.5% Serum Kdo2-Lipid A TreatmentPP0000000500Added 08/27/07
0.5% Serum Kdo2-Lipid A TreatmentPP0000000501Added 08/27/07

Primary Macrophages

Eliciting Bio-Gel Derived MacrophagesPP0000001300Added 08/27/07
Eliciting Foam CellsPP0000003000Added 08/22/06
Eliciting Thioglycollate Derived MacrophagesPP0000001400Added 08/27/07
Eliciting Thioglycollate derived MacrophagesPP0000001401Added 06/20/07
Harvesting and Plating Bone Marrow-derived MacrophagesPP0000003500Added 06/20/07
Harvesting and Plating Primary MacrophagesPP0000001501 
Harvesting and Plating Thioglycollate Elicited MacrophagesPP0000001502Added 06/20/07
Harvesting Foam CellsPP0000003100Added 08/22/06
Harvesting MacrophagesPP0000001500Added 08/27/07
Kdo2-Lipid A Treatment of Thioglycollate and Bone Marrow Derived MacrophagesPP0000001801Added 06/20/07
Primary Macrophage TreatmentPP0000001800 

Core D - Macrophage Biology and Functional Genomics

Core D - Procedure Protocol Compactin, Kdo2 Lipid A Treatment 2008PP0000004000Added 10/09/12
Core D - 25OHC TreatmentPP0000004900Added 11/24/10

Core E - Glycerolipids/Cholesteryl Esters

Core E - LM Thawing and Passage for RAW 264.7PP0000001900Added 08/27/07
Core E - 0.5% Serum Kdo2-Lipid A TreatmentPP0000002000Added 08/27/07
Core E - 10% Serum Kdo2-Lipid A TreatmentPP0000002100Added 08/27/07
Core E - Analysis of Neutral LipidsPP0000002200Added 08/27/07
Core E - Analysis of Neutral Lipids from Human Plasma June 4, 2010PP0000005000Added 10/09/12
Core E - Analysis of Neutral Lipids from Cells June 4, 2010PP0000005500Added 10/09/12

Core G - Eicosanoids

Core G - Eicosanoid MSPP0000000200Added 08/27/07
Core G - Eicosanoid MSPP0000000201Added 08/27/07
Core G - Eicosanoid MSPP0000000204Added 08/27/07
Core G - Eicosanoid MSPP0000000205Added 08/27/07
Core G - Eicosanoid MSPP0000000206Added 08/27/07
Core G - RAW subcellular fractionationPP0000004300Added 05/19/08
Core G - RAW subcellular fractionationPP0000004301Added 06/10/08

Core H - Glycerophospholipids

Core H - Direct Injection MSPP0000000900Added 08/27/07
Core H - HPLC phospholipid protocolPP0000000800Added 08/27/07
Phospholipid LC-MS ParametersPP0000002600Added 08/27/07
Core H - Glycerophospholipid ExtractionPP0000004200Added 04/23/08

Core J - Sterols

Core J - 10% NCLPPS, Compactin, Kdo2-Lipid A TreatmentPP0000002300Added 08/27/07
Core J - Compactin Kdo2-Lipid A Treatment in Dye-Free GMedPP0000002400Added 08/27/07
Core J - Sterol Extraction and LCMSPP0000002500Added 08/27/07
Core J - Compactin Kdo2-Lipid A TreatmentPP0000002800Added 08/27/07
Core J - Compactin Kdo2-Lipid A ModifiedPP0000003300Added 08/27/07
Core J - Compactin Kdo2lipidA RNAPP0000003400Added 08/27/07
Core J - Sterol Extraction and QuantitationPP0000003200Added 08/27/07
Core J - Sterol Extraction and QuantitationPP0000003201Added 08/27/07
Core J - Foam Cell Sterol Extraction and QuantitationPP0000003600Added 05/21/07
Core J - Sterol Extraction, Cleanup, and QuantitationPP0000003700Added 05/21/07
Core J - Procedure Protocol - Compactin, Kdo2 Lipid A Treatment 2008PP0000003900Added 11/22/10
Core J - Procedure Protocol Compactin, Kdo2 Lipid A Treatment in Dye-Free Growth Medium 2008PP0000004400Added 10/09/12
Core J - Human Plasma Samples First Set of Practice and NIST PlasmasPP0000004500Added 10/09/12

Core K - Prenols and Other Lipids

Core K - Human plasma SRM neutral folch extraction and lipid analysisPP0000004800Added 10/09/12

Key LIPID MAPS® Resources
Publications
- publications related to projects funded through the LIPID MAPS® consortium
Lipid Classification System
- the first internationally accepted lipid classification system
Lipid Standards
- MS/MS spectra, annotations for principal product ions, and acquisition parameters
Experimental Data
- centralized studies on macrophages, lipidomics data on human plasma, and more
Structure Database (LMSD)
- over 40,000 unique structures of biologically relevant lipids
Proteome Database (LMPD)
- over 12,500 lipid-associated proteins from major research species
Pathways
- manually curated lipid metabolism and signaling pathways
LIPID MAPS® REST Service
- web-based/programmatic access to lipid structure and lipid-related gene/protein data
MS analysis tools
- tools for searching various lipid classes by precursor or product ion
Structure Drawing Tools
- draw and save lipid structures using online menus
Protocols
- LIPID MAPS® techniques for lipidomics and macrophage cell culture

LIPID MAPS® is funded by a grant from the Wellcome Trust.

logo LIPID MAPS® is funded by a grant from the Wellcome Trust.