Lipidomics methods

  • Overview of Quantitative Lipid Analysis by Mass Spectrometry (png) (PDF) - Flow chart outlining the procedure for lipid analysis of various lipid classes, from extraction to laboratory analysis to bioinformatics.
  • Chemical Reviews 111 (2011), Special issue: Lipid Biochemistry Metabolism and Signaling Edited by H. Alex Brown and Lawrence J. Marnett
  • BBA - Molecular and Cellular Biology of Lipids 1811 (2011), Special issue: Lipidomics and Imaging Mass Spectrometry Edited by Alfred H. Merrill and Robert C. Murphy
  • Methods in Enzymology, Academic Press 432 (2007), LIPID MAPS chapters - (PDF). For the complete Methods in Enzymology volumes, including LIPID MAPS chapters in Volumes 432, 433, and 434, please see http://www.sciencedirect.com/science/bookseries/00766879. References to individual chapters from these volumes can also be found on our LIPID MAPS Publications: 2007 page.
    • Qualitative Analysis and Quantitative Assessment of Changes in Neutral Glycerol Lipid Molecular Species Within Cells
    • Glycerophospholipid Identification and Qualitation by Electrospray Ionization Mass Spectrometry
    • Detection and Quantitation of Eicosanoids via High Performance Liquid Chromotography-Electrospray Ionization-Mass Spectrometry
    • Structure-Specific, Quantitative Methods for Analysis of Sphingolipids by Liquid Chromotography-Tandem Mass Spectrometry: "Inside-Out" Sphingolipidomics
    • Analysis of Ubiquinones, Dolichols, and Dolichol Diphosphate-Oligosaccharides by Liquid Chromatography-Electrospray Ionization-Mass Spectrometry
    • Extraction and Analysis of Sterols in Biological Matrices by High Performance Liquid Chromatography Electrospray Ionization Mass Spectrometry
    • The Lipid Maps Initiative in Lipidomics
    • Bioinformatics for Lipidomics
    • Quantitation and Standardization of Lipid Internal Standards for Mass Spectrometry
  • Lipidomics workshop at EB2009 - (PDFs) The LIPID MAPS Consortium presented a workshop on lipidomics techniques at EB2009 under the auspices of the ASBMB. The slides from each of these presentations contain references to most of the methodoligies described .
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