The LIPID MAPS Webinar Series will feature presentations from several lipidomics experts who will introduce basic features related to structure, function, and metabolism, and discuss biochemistry and analysis of various lipid classes.
Synopsis: This webinar will give an introductory overview of the structures, metabolic pathways and functions of sphingolipids and glycosphingolipids, then it will expand on this familiar framework to the much more complex picture that emerges when the entire "(glyco)sphingolipidome" is considered. Where known, the origins of the structural diversity will be discussed, as well as some of the consequences for biologic function and disease. An understanding of these topics will be useful in the design and interpretation of lipidomic analyses that include (glyco)sphingolipids.
Synopsis: This webinar will give an overview of methods for lipidomic analysis of sphingolipids and glycosphingolipids, from classical techniques to mass spectrometry, including imaging MS. The discussion will encompass:extraction and solubilization; selection of standards and quantitation; complications due to isomers and isobars; the use, and limitations, of isotopically labeled precursors for estimation of metabolic flux; complementary tools that can provide insight into lipidomic data; and ways of displaying (glyco)sphingolipidomic results. An understanding of these topics will be useful in the design and interpretation of lipidomic analyses that include (glyco)sphingolipids.
Al has studied sphingolipids for nearly forty years and his laboratory and collaborators have contributed to our understanding of sphingolipid structure and function, metabolism, and interrelationships between sphingolipids and disease. He and Dr. Cameron Sullards, who is now with Avanti Polar Lipids, were the directors of the Sphingolipid and Glycosphingolipid Core Laboratory for the first decade of LIPID MAPS, and he now serves as a member of the Scientific Advisory Committee.