LMPD Database

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LMP002728

UniProt Annotations

Entry Information
Gene NameH2-K region expressed gene 6
Protein EntryDHB8_MOUSE
UniProt IDP50171
SpeciesMouse
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=2; Name=Short; IsoId=P50171-1; Sequence=Displayed; Name=Long; IsoId=P50171-2; Sequence=VSP_006030;
Biophysicochemical PropertiesKinetic parameters: KM=0.110 uM for estradiol {ECO:0000269|PubMed:9712896}; KM=0.422 uM for testosterone {ECO:0000269|PubMed:9712896}; KM=0.368 uM for estrone {ECO:0000269|PubMed:9712896}; KM=0.360 uM for dihydrotestosterone {ECO:0000269|PubMed:9712896}; Vmax=0.405 nmol/min/mg enzyme for estradiol as substrate {ECO:0000269|PubMed:9712896}; Vmax=0.123 nmol/min/mg enzyme for testosterone as substrate {ECO:0000269|PubMed:9712896}; Vmax=0.186 nmol/min/mg enzyme for estrone as substrate {ECO:0000269|PubMed:9712896}; Vmax=0.081 nmol/min/mg enzyme for dihydrotestosterone as substrate {ECO:0000269|PubMed:9712896};
Catalytic Activity17-beta-estradiol + NAD(P)(+) = estrone + NAD(P)H. {ECO:0000269|PubMed:9712896}.
Catalytic ActivityTestosterone + NAD(+) = androstenedione + NADH. {ECO:0000269|PubMed:9712896}.
FunctionNAD-dependent 17-beta-hydroxysteroid dehydrogenase with highest activity towards estradiol. Has very low activity towards testosterone (By similarity). The heteroteramer with CBR4 has NADH-dependent 3-ketoacyl-acyl carrier protein reductase activity. May play a role in biosynthesis of fatty acids in mitochondria (By similarity). {ECO:0000250}.
PathwayLipid metabolism; fatty acid biosynthesis.
PathwaySteroid biosynthesis; estrogen biosynthesis.
Sequence CautionSequence=AAC69902.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
SimilarityBelongs to the short-chain dehydrogenases/reductases (SDR) family. {ECO:0000305}.
Subcellular LocationMitochondrion matrix {ECO:0000250}.
SubunitHeterotetramer with CBR4; contains two molecules of HSD17B8 and CBR4. {ECO:0000250}.
Tissue SpecificityKidney, liver, testis, ovary, oviduct, uterus, mammary gland, vagina, prostate, clitoral gland and moderately in spleen, heart, dorsal skin, brain and lung.