LMPD Database

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LMP012893

UniProt Annotations

Entry Information
Gene Nameacyl-CoA synthetase long-chain family member 6
Protein EntryACSL6_RAT
UniProt IDP33124
SpeciesRat
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=2; Name=1; Synonyms=ACSL6_vs1; IsoId=P33124-1; Sequence=Displayed; Note=Contains exon 14; Name=2; Synonyms=ACSL6_vs2; IsoId=P33124-2; Sequence=VSP_057107, VSP_057108; Note=Contains exon 13. No differences in the affinity for fatty acids and CoA. Decreased affinity for ATP. Could play an important role at lower ATP levels. Expressed at lower level in the brain compared to isoform 1. ;
Biophysicochemical PropertiesKinetic parameters: KM=12210 uM for ATP (Isoform 1) ; KM=4.7 uM for CoA (Isoform 1) ; KM=6.0 uM for palmitate (Isoform 1) ; KM=3.0 uM for oleate (Isoform 1) ; KM=9.7 uM for arachidonate (Isoform 1) ; KM=1480 uM for ATP (Isoform 2) ; KM=2.4 uM for CoA (Isoform 2) ; KM=3.6 uM for palmitate (Isoform 2) ; KM=5.3 uM for oleate (Isoform 2) ; KM=6.5 uM for arachidonate (Isoform 2) ; Vmax=208 nmol/min/mg enzyme with palmitate as substrate (Isoform 1) ; Vmax=153 nmol/min/mg enzyme with oleate as substrate (Isoform 1) ; Vmax=139 nmol/min/mg enzyme with arachidonate as substrate (Isoform 1) ; Vmax=739 nmol/min/mg enzyme with palmitate as substrate (Isoform 2) ; Vmax=1088 nmol/min/mg enzyme with oleate as substrate (Isoform 2) ; Vmax=1212 nmol/min/mg enzyme with arachidonate as substrate (Isoform 2) ;
Catalytic ActivityATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.
CofactorName=Mg(2+); Xref=ChEBI:CHEBI:18420;
Developmental StageIsoform 1 is detected 10 days after birth, and increased in a coordinate fashion during the development of brain, and the amount did not decrease when myelination was completed
FunctionActivation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Plays an important role in fatty acid metabolism in brain and the acyl-CoAs produced may be utilized exclusively for the synthesis of the brain lipid.
Miscellaneous5 rat isozymes encoded by different genes have been described. ACSL6 corresponds to isozyme 2 (ACS2)
SimilarityBelongs to the ATP-dependent AMP-binding enzyme family
Subcellular LocationMitochondrion outer membrane ; Single-pass type III membrane protein . Peroxisome membrane ; Single-pass type III membrane protein {ECO:0000250}. Microsome membrane ; Single-pass type III membrane protein . Endoplasmic reticulum membrane ; Single-pass type III membrane protein .
Tissue SpecificityExpressed predominantly in brain and, to a much lesser extent, in heart and adrenal.