Lipidomics studies on macrophages - Foam cell study with wild-type and LDLR(-/-) mice, normal and high-fat diets

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Legend:graph_icon: View data for technical replicates
graph icon: Graph of technical replicates(within an expt.) graph icon: Graph of biological replicates(across expts.)
Details/Graph
technical replicates
LM_ID NameMass
(neutral)
WT-Fat
WT-Chow
KO-Fat
KO-Chow
Units
. . . .
(MSJ070927)LMST01010086 22R-hydroxy-cholesterol402.3 (ions) 6.8
-
15
6.7
pmol/1E6 cells
(MSJ070927)LMST01010018 25-hydroxy-cholesterol402.3 (ions) 1.8
-
4.3
1.7
pmol/1E6 cells
(MSJ070927)LMST01010057 27-hydroxy-cholesterol402.3 (ions) 1
-
2
3.3
pmol/1E6 cells
(MSJ070927)LMST01010014 4beta-hydroxy-cholesterol402.3 (ions) 3.5
4
4.2
5.2
pmol/1E6 cells
(MSJ070927)LMST01010013 7alpha-hydroxy-cholesterol402.3 (ions) 6.9
3.3
11
5.3
pmol/1E6 cells
(MSJ070927)LMST01010069 7-Dehydrocholesterol384.3 (ions) -
-
-
-
pmol/1E6 cells
(MSJ070927)LMST01010049 7-oxo-cholesterol400.3 (ions) -
-
-
-
pmol/1E6 cells
(MSJ070927)LMST01010015 Cholestenone384.3 (ions) 0.7
0.53
-
-
pmol/1E6 cells
(MSJ070927)LMST01010001 Cholesterol386.4 (ions) 9890
8860
32720
13780
pmol/1E6 cells
(MSJ070927)LMST01010017 Lanosterol426.4 (ions) 3.3
2.5
5
2.5
pmol/1E6 cells

 


*: The units in the table and graphs correspond to the pmol of each species which was determined by dividing the areas under the LC MS/MS elution curve for each species by that for an appropriate deuterated surrogate standard then multiplied by the amount of the surrogate standard added (~10 pmol to 2 nmol depending on the compound of interest). For most of the analytes, the intensities were similiar for equivalent amounts of the analyte as for the internal standard when the LC MS/MS parameters were optimized and quantitative analysis was conducted by Multiple Reaction Monitoring (MRM). If necessary, small corrections were made using empirically determined relative response factors (RRF). The pmoles were then normalized to the measured quantity of DNA present in each sample (i.e. dish of RAW cells at time zero or at varying times following treatment with and without Kdo2 lipid A).