Lipidomics studies on macrophages - Foam cell study with wild-type and LDLR(-/-) mice, normal and high-fat diets

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Legend:graph_icon: View data for technical replicates
graph icon: Graph of technical replicates(within an expt.) graph icon: Graph of biological replicates(across expts.)
Details/Graph
technical replicates
LM_ID NameMass
(neutral)
WT-Fat
WT-Chow
KO-Fat
KO-Chow
Units
. . . .
(MSG070316)LMFA01030283 10Z-heptadecenoic acid268.2 (ions) 9.7
0.93
14
4.9
pmol/1E6 cells
(MSG070316)LMFA01030159 11,14,17-eicosatrienoic acid306.3 (ions) 89
26
170
45
pmol/1E6 cells
(MSG070316)LMFA01030130 11,14-eicosadienoic acid308.3 (ions) 81
220
120
290
pmol/1E6 cells
(MSG070316)LMFA01030408 13,16,19-docosatrienoic acid334.3 (ions) 2.1
2
3.9
3.1
pmol/1E6 cells
(MSG070316)LMFA01030132 13,16-docosadienoic acid336.3 (ions) 22
75
37
110
pmol/1E6 cells
(MSG070316)LMFA01030759 5,8,11,14,17-eicosapentaenoic acid302.2 (ions) 110
13
280
27
pmol/1E6 cells
(MSG070316)LMFA01030157 5,8,11-eicosatrienoic acid306.3 (ions) 120
98
150
160
pmol/1E6 cells
(MSG070316)LMFA01030184 7,10,13,16,19-docosapentaenoic acid330.3 (ions) 290
72
410
120
pmol/1E6 cells
(MSG070316)LMFA01030178 7Z,10Z,13Z,16Z-docosatetraenoic acid332.3 (ions) 100
240
150
390
pmol/1E6 cells
(MSG070316)LMFA01030056 9Z-palmitoleic acid254.2 (ions) 680
120
850
200
pmol/1E6 cells
(MSG070316)LMFA01030152 alpha-linolenic acid278.2 (ions) 2.1
1.7
3.7
2.7
pmol/1E6 cells
(MSG070316)LMFA01010020 Arachidic acid312.3 (ions) 24
17
34
26
pmol/1E6 cells
(MSG070316)LMFA01030001 Arachidonic acid304.2 (ions) 340
370
470
590
pmol/1E6 cells
(MSG070316)LMFA01010022 Behenic acid340.3 (ions) 3.8
2.1
7.1
3.5
pmol/1E6 cells
(MSG070316)LMFA01030158 bishomo-gamma-linolenic acid306.3 (ions) 19
11
34
16
pmol/1E6 cells
(MSG070316)LMFA01010026 Cerotic acid396.4 (ions) 0.7
0.6
1.5
0.63
pmol/1E6 cells
(MSG070316)LMFA01030089 cis-erucic acid338.3 (ions) 84
51
120
71
pmol/1E6 cells
(MSG070316)LMFA01030092 cis-selacholeic acid366.3 (ions) 4.5
3.1
9.2
4.8
pmol/1E6 cells
(MSG070316)LMFA01030185 DHA328.2 (ions) 350
150
550
260
pmol/1E6 cells
(MSG070316)LMFA01030141 gamma-linolenic acid278.2 (ions) 55
30
75
39
pmol/1E6 cells
(MSG070316)LMFA01010012 Lauric acid200.2 (ions) 23
11
31
9.1
pmol/1E6 cells
(MSG070316)LMFA01010024 Lignoceric acid368.4 (ions) 5.6
3.8
12
5.9
pmol/1E6 cells
(MSG070316)LMFA01030120 Linoleic acid280.2 (ions) 580
810
780
1050
pmol/1E6 cells
(MSG070316)LMFA01010017 Margaric acid270.3 (ions) 100
43
140
59
pmol/1E6 cells
(MSG070316)LMFA01010014 Myristic acid228.2 (ions) 340
99
430
130
pmol/1E6 cells
(MSG070316)LMFA01030002 Oleic acid282.3 (ions) 1330
1020
1270
1110
pmol/1E6 cells
(MSG070316)LMFA01010001 Palmitic acid256.2 (ions) 2400
1130
2220
1410
pmol/1E6 cells
(MSG070316)LMFA01010015 Pentadecanoic acid242.2 (ions) 130
25
180
31
pmol/1E6 cells
(MSG070316)LMFA01010018 Stearic acid284.3 (ions) 1160
840
1200
1120
pmol/1E6 cells
(MSG070316)LMFA01030357 Stearidonic acid276.2 (ions) 0.33
0.1
0.53
0.1
pmol/1E6 cells
(MSG070316)LMFA01010023 Tricosanoic acid354.3 (ions) 2.3
0.93
4
1.5
pmol/1E6 cells

 


*:The units in the table and graphs correspond to the intensity of each analyte divided by the intensity of the corresponding deuterated internal standard. The amount of internal standard added to each sample in the time series and control experiments was identical. The relative intensity values were converted to pmoles by extrapolating from the standard curve for that analyte and then normalized to the number of cells in a given sample as measured by the quantity of DNA in the well.