Lipidomics studies on macrophages - Foam cell study with wild-type and LDLR(-/-) mice, normal and high-fat diets

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Legend:graph_icon: View data for technical replicates
graph icon: Graph of technical replicates(within an expt.) graph icon: Graph of biological replicates(across expts.)
Details/Graph
technical replicates
LM_ID NameMass
(neutral)
WT-Fat
WT-Chow
KO-Fat
KO-Chow
Units
. . . .
(MSG070316)LMFA01030283 10Z-heptadecenoic acid268.2 (ions) 7.8
0.83
13
0.73
pmol/1E6 cells
(MSG070316)LMFA01030159 11,14,17-eicosatrienoic acid306.3 (ions) 88
25
170
38
pmol/1E6 cells
(MSG070316)LMFA01030130 11,14-eicosadienoic acid308.3 (ions) 72
210
94
320
pmol/1E6 cells
(MSG070316)LMFA01030408 13,16,19-docosatrienoic acid334.3 (ions) 2.5
2
2.7
3.4
pmol/1E6 cells
(MSG070316)LMFA01030132 13,16-docosadienoic acid336.3 (ions) 23
74
24
120
pmol/1E6 cells
(MSG070316)LMFA01030759 5,8,11,14,17-eicosapentaenoic acid302.2 (ions) 86
12
270
20
pmol/1E6 cells
(MSG070316)LMFA01030157 5,8,11-eicosatrienoic acid306.3 (ions) 100
93
110
140
pmol/1E6 cells
(MSG070316)LMFA01030184 7,10,13,16,19-docosapentaenoic acid330.3 (ions) 250
70
320
110
pmol/1E6 cells
(MSG070316)LMFA01030178 7Z,10Z,13Z,16Z-docosatetraenoic acid332.3 (ions) 99
230
120
360
pmol/1E6 cells
(MSG070316)LMFA01030056 9Z-palmitoleic acid254.2 (ions) 510
120
960
160
pmol/1E6 cells
(MSG070316)LMFA01030152 alpha-linolenic acid278.2 (ions) 1.7
1.9
3.5
2.4
pmol/1E6 cells
(MSG070316)LMFA01010020 Arachidic acid312.3 (ions) 20
17
24
23
pmol/1E6 cells
(MSG070316)LMFA01030001 Arachidonic acid304.2 (ions) 260
360
460
530
pmol/1E6 cells
(MSG070316)LMFA01010022 Behenic acid340.3 (ions) 3.1
2.4
5.2
4
pmol/1E6 cells
(MSG070316)LMFA01030158 bishomo-gamma-linolenic acid306.3 (ions) 17
10
29
17
pmol/1E6 cells
(MSG070316)LMFA01010026 Cerotic acid396.4 (ions) 0.57
0.7
0.8
0.83
pmol/1E6 cells
(MSG070316)LMFA01030089 cis-erucic acid338.3 (ions) 77
51
82
72
pmol/1E6 cells
(MSG070316)LMFA01030092 cis-selacholeic acid366.3 (ions) 4.4
3.5
6.1
5.2
pmol/1E6 cells
(MSG070316)LMFA01030185 DHA328.2 (ions) 300
150
490
260
pmol/1E6 cells
(MSG070316)LMFA01030141 gamma-linolenic acid278.2 (ions) 38
26
92
36
pmol/1E6 cells
(MSG070316)LMFA01010012 Lauric acid200.2 (ions) 20
11
47
4.9
pmol/1E6 cells
(MSG070316)LMFA01010024 Lignoceric acid368.4 (ions) 5
4.4
7.3
6.8
pmol/1E6 cells
(MSG070316)LMFA01030120 Linoleic acid280.2 (ions) 440
730
830
1030
pmol/1E6 cells
(MSG070316)LMFA01010017 Margaric acid270.3 (ions) 80
44
120
50
pmol/1E6 cells
(MSG070316)LMFA01010014 Myristic acid228.2 (ions) 260
100
440
120
pmol/1E6 cells
(MSG070316)LMFA01030002 Oleic acid282.3 (ions) 1200
960
1300
1090
pmol/1E6 cells
(MSG070316)LMFA01010001 Palmitic acid256.2 (ions) 1650
1040
2520
1280
pmol/1E6 cells
(MSG070316)LMFA01010015 Pentadecanoic acid242.2 (ions) 98
24
180
30
pmol/1E6 cells
(MSG070316)LMFA01010018 Stearic acid284.3 (ions) 840
800
1120
960
pmol/1E6 cells
(MSG070316)LMFA01030357 Stearidonic acid276.2 (ions) 0.23
0.1
0.63
0.1
pmol/1E6 cells
(MSG070316)LMFA01010023 Tricosanoic acid354.3 (ions) 1.5
1.1
3
1.5
pmol/1E6 cells

 


*:The units in the table and graphs correspond to the intensity of each analyte divided by the intensity of the corresponding deuterated internal standard. The amount of internal standard added to each sample in the time series and control experiments was identical. The relative intensity values were converted to pmoles by extrapolating from the standard curve for that analyte and then normalized to the number of cells in a given sample as measured by the quantity of DNA in the well.