Lipidomics studies on macrophages - Foam cell study with wild-type and LDLR(-/-) mice, normal and high-fat diets

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Legend:graph_icon: View data for technical replicates
graph icon: Graph of technical replicates(within an expt.) graph icon: Graph of biological replicates(across expts.)
PA| PC| PE| PG| PI| PS

Details/Graph
technical replicates
LM_ID NameMass
(neutral)
WT-Fat
WT-Chow
KO-Fat
KO-Chow
Units
. . . .
(BCJ070122) PA(32:0)648.5 (ions) 4.7
12
6.1
8.8
pmol/1E6 cells
(BCJ070122) PA(32:1)646.5 (ions) 3.4
2
5
2
pmol/1E6 cells
(BCJ070122) PA(34:0)676.5 (ions) 4.2
11
7.3
7.2
pmol/1E6 cells
(BCJ070122) PA(34:1)674.5 (ions) 20
23
17
16
pmol/1E6 cells
(BCJ070122) PA(34:2)672.5 (ions) 4.8
6.4
4.3
4.4
pmol/1E6 cells
(BCJ070122) PA(36:0)704.5 (ions) 4.5
4.9
3.4
3.6
pmol/1E6 cells
(BCJ070122) PA(36:1)702.5 (ions) 20
28
19
20
pmol/1E6 cells
(BCJ070122) PA(36:2)700.5 (ions) 24
38
17
26
pmol/1E6 cells
(BCJ070122) PA(36:3)698.5 (ions) 5.2
7
5.1
4.8
pmol/1E6 cells
(BCJ070122) PA(36:4)696.5 (ions) 3.2
4.5
2.7
4.2
pmol/1E6 cells
(BCJ070122) PA(38:2)728.5 (ions) 2.6
2.8
1.8
1.8
pmol/1E6 cells
(BCJ070122) PA(38:3)726.5 (ions) 8
9.8
7.8
7.5
pmol/1E6 cells
(BCJ070122) PA(38:4)724.5 (ions) 12
23
15
21
pmol/1E6 cells
(BCJ070122) PA(40:3)754.6 (ions) 1.5
5.9
2.3
3.8
pmol/1E6 cells
(BCJ070122) PA(40:4)752.5 (ions) 5
25
6.4
25
pmol/1E6 cells
(BCJ070122) PA(40:5)750.5 (ions) 13
15
14
15
pmol/1E6 cells
(BCJ070122) PA(40:6)748.5 (ions) 9.9
14
15
10
pmol/1E6 cells

 


*:The units in the table and graphs correspond to the intensity of each species divided by the average intensity (peak area) of a set of odd-chain internal standards corresponding to that particular headgroup. No internal standards were available for the PI series so intensity of each species was divided by the the total PI intensity in this case. The amount of internal standard added to each sample in the time series and control experiments was identical. The relative intensity values were then normalized to the measured quantity of DNA present in each RAW cell preparation following treatment with and without Kdo2 lipid A for each time point.