LIPID MAPS

Resolvin E2

LMFA03140011

Thermo QExactive HF

[M-H]-

N.Kampschulte, M.A.Chromik, R.Kirchhoff, A.Löwen, K.Mosel, K.Plitzko, L.Scholz, L.Wende, M.Wiebel, N.H.Schebb

Structure

Common name

Resolvin E2

LMID

LMFA03140011

Systematic name

5S,18R-dihydroxy-6E,8Z,11Z,14Z,16E-eicosapentaenoic acid

m/z

333.2

Adduct / Ion

[M-H]-

Charge

-1

Ion source

HESI

Instrument

Thermo QExactive HF

Resolution

Not Available

Ion mode

Negative

Collision Energy (CE)

30

Column

Zorbax Eclipse Plus C18 reversed phase column (2.1 x 150 mm, particle size 1.8 mm; Agilent, Waldbronn, Germany)

Mobile phase A

95/5 (v/v) 0.1% acetic acid (aq)/Eluent B

Mobile phase B

800/150/1 (v/v/v) acetonitrile/methanol/acetic acid

Retention time (min)

11.81

Amount injected

~ 0.3 ng

DOI for method

10.1016/j.aca.2017.11.002

Date acquired

February 2025

Submitters

N.Kampschulte, M.A.Chromik, R.Kirchhoff, A.Löwen, K.Mosel, K.Plitzko, L.Scholz, L.Wende, M.Wiebel, N.H.Schebb

Source of standard

Cayman Chemical

Resolvin E2

LMFA03140011

Sciex 5500, QqQ

[M-H]-

N.Kampschulte, M.A.Chromik, R.Kirchhoff, A.Löwen, K.Mosel, K.Plitzko, L.Scholz, L.Wende, M.Wiebel, N.H.Schebb

Structure

Common name

Resolvin E2

LMID

LMFA03140011

Systematic name

5S,18R-dihydroxy-6E,8Z,11Z,14Z,16E-eicosapentaenoic acid

m/z

333.2

Adduct / Ion

[M-H]-

Charge

-1

Ion source

ESI

Instrument

Sciex 5500, QqQ

Resolution

N/A

Ion mode

Negative

Collision Energy (CE)

-15 - -25

Column

Zorbax Eclipse Plus C18 reversed phase column (2.1 x 150 mm, particle size 1.8 mm; Agilent, Waldbronn, Germany)

Mobile phase A

95/5 (v/v) 0.1% acetic acid (aq)/Eluent B

Mobile phase B

800/150/1 (v/v/v) acetonitrile/methanol/acetic acid

Retention time (min)

11.81

Amount injected

~ 0.3 ng

DOI for method

10.1016/j.aca.2017.11.002

Date acquired

April 2025

Submitters

N.Kampschulte, M.A.Chromik, R.Kirchhoff, A.Löwen, K.Mosel, K.Plitzko, L.Scholz, L.Wende, M.Wiebel, N.H.Schebb

Source of standard

Cayman Chemical

Resolvin E2

LMFA03140011

Sciex 6500+/QTrap

[M-H]-

N.Kampschulte, M.A.Chromik, R.Kirchhoff, A.Löwen, K.Mosel, K.Plitzko, L.Scholz, L.Wende, M.Wiebel, N.H.Schebb

Structure

Common name

Resolvin E2

LMID

LMFA03140011

Systematic name

5S,18R-dihydroxy-6E,8Z,11Z,14Z,16E-eicosapentaenoic acid

m/z

333.2

Adduct / Ion

[M-H]-

Charge

-1

Ion source

ESI

Instrument

Sciex 6500+/QTrap

Resolution

N/A

Ion mode

Negative

Collision Energy (CE)

-15 - -25

Column

Zorbax Eclipse Plus C18 reversed phase column (2.1 x 150 mm, particle size 1.8 mm; Agilent, Waldbronn, Germany)

Mobile phase A

95/5 (v/v) 0.1% acetic acid (aq)/Eluent B

Mobile phase B

800/150/1 (v/v/v) acetonitrile/methanol/acetic acid

Retention time (min)

11.81

Amount injected

~ 0.3 ng

DOI for method

10.1016/j.aca.2017.11.002

Date acquired

February 2025

Submitters

N.Kampschulte, M.A.Chromik, R.Kirchhoff, A.Löwen, K.Mosel, K.Plitzko, L.Scholz, L.Wende, M.Wiebel, N.H.Schebb

Source of standard

Cayman Chemical

Resolvin E2

LMFA03140011

Waters Xevo TQ-XS Triple Quadrupole

[M-H]-

Wheelock/Hagn

Structure

Common name

Resolvin E2

LMID

LMFA03140011

Systematic name

5S,18R-dihydroxy-6E,8Z,11Z,14Z,16E-eicosapentaenoic acid

m/z

333.2

Adduct / Ion

[M-H]-

Charge

-1

Ion source

ESI

Instrument

Waters Xevo TQ-XS Triple Quadrupole

Resolution

N/A

Ion mode

Negative

Collision Energy (CE)

15

Column

Waters Acquity Premier BEH C18 with VanGuard FIT, 130 Å, 1.7 µm, 2.1 x 100 mm

Mobile phase A

Milli-Q H2O + 0.1 % (v/v) acetic acid

Mobile phase B

ACN:IPA (90:10)

Retention time (min)

4.33

Amount injected

0.25 ng

DOI for method

10.1021/acs.analchem.8b01651

Date acquired

March 2025

Submitters

Wheelock/Hagn

Source of standard

Cayman Chemical