LIPID MAPS

17(RS)-10-epi-SC-D15-11-dihomo-IsoF

LMFA04020529

Thermo Scientific Q Exactive Focus Orbitrap LC-MS/MS System

[M-H]-

Jean-Marie Galano.Ángel Sánchez Illana.Thierry Durand

Structure

Common name

17(RS)-10-epi-SC-D15-11-dihomo-IsoF

LMID

LMFA04020529

Systematic name

(R,Z)-10-hydroxy-10-((2S,4S,5S)-4-hydroxy-5-((E)-3-hydroxyoct-1-en-1-yl)tetrahydrofuran-2-yl)dec-7-enoic acid

m/z

397.2594

Adduct / Ion

[M-H]-

Charge

-1

Ion source

ESI

Instrument

Thermo Scientific Q Exactive Focus Orbitrap LC-MS/MS System

Resolution

17000

Ion mode

Negative

Collision Energy (CE)

30eV

Column

Kinetex EVO C18 (100 × 2.1 mm column 1.7 µm)

Mobile phase A

0.3 mL/min binary gradient using H2O (0.1% HCOOH)

Mobile phase B

CH3CN:CH3OH 8:2, (0.1% HCOOH)

Retention time (min)

13.97

Amount injected

5μl

DOI for method

10.1038/s41597-024-03034-4

Date acquired

October 2023

Submitters

Jean-Marie Galano.Ángel Sánchez Illana.Thierry Durand

Source of standard

Synthesized in-house

17(RS)-10-epi-SC-D15-11-dihomo-IsoF

LMFA04020529

Thermo Fisher Orbitrap ID-X Tribrid

[M-H]-

Jean-Marie Galano.Ángel Sánchez Illana.Thierry Durand

Structure

Common name

17(RS)-10-epi-SC-D15-11-dihomo-IsoF

LMID

LMFA04020529

Systematic name

(R,Z)-10-hydroxy-10-((2S,4S,5S)-4-hydroxy-5-((E)-3-hydroxyoct-1-en-1-yl)tetrahydrofuran-2-yl)dec-7-enoic acid

m/z

397.2595

Adduct / Ion

[M-H]-

Charge

-1

Ion source

ESI

Instrument

Thermo Fisher Orbitrap ID-X Tribrid

Resolution

60000

Ion mode

Negative

Collision Energy (CE)

stepped HCD collision energy 20–40–60%

Column

Kinetex C18 (100 × 2.1 mm, 1.7 µm)

Mobile phase A

H2O (0.1% HCOOH)

Mobile phase B

CH3CN (0.1% HCOOH) binary gradient at 0.5 m L/min

Retention time (min)

6.25

Amount injected

10 µL

DOI for method

10.1038/s41597-024-03034-4

Date acquired

October 2023

Submitters

Jean-Marie Galano.Ángel Sánchez Illana.Thierry Durand

Source of standard

Synthesized in-house

17(RS)-10-epi-SC-D15-11-dihomo-IsoF

LMFA04020529

Sciex 5500, QqQ

[M-H]-

N.Kampschulte, M.A.Chromik, R.Kirchhoff, A.Löwen, K.Mosel, K.Plitzko, L.Scholz, L.Wende, M.Wiebel, N.H.Schebb

Structure

Common name

17(RS)-10-epi-SC-D15-11-dihomo-IsoF

LMID

LMFA04020529

Systematic name

(R,Z)-10-hydroxy-10-((2S,4S,5S)-4-hydroxy-5-((E)-3-hydroxyoct-1-en-1-yl)tetrahydrofuran-2-yl)dec-7-enoic acid

m/z

397.3

Adduct / Ion

[M-H]-

Charge

-1

Ion source

ESI

Instrument

Sciex 5500, QqQ

Resolution

N/A

Ion mode

Negative

Collision Energy (CE)

-15 - -25

Column

Zorbax Eclipse Plus C18 reversed phase column (2.1 x 150 mm, particle size 1.8 mm; Agilent, Waldbronn, Germany)

Mobile phase A

95/5 (v/v) 0.1% acetic acid (aq)/Eluent B

Mobile phase B

800/150/1 (v/v/v) acetonitrile/methanol/acetic acid

Retention time (min)

11.85

Amount injected

~ 0.3 ng

DOI for method

10.1016/j.aca.2017.11.002

Date acquired

April 2025

Submitters

N.Kampschulte, M.A.Chromik, R.Kirchhoff, A.Löwen, K.Mosel, K.Plitzko, L.Scholz, L.Wende, M.Wiebel, N.H.Schebb

Source of standard

chemical synthesis by T. Durand and Coworkers

17(RS)-10-epi-SC-D15-11-dihomo-IsoF

LMFA04020529

Sciex 6500+/QTrap

[M-H]-

N.Kampschulte, M.A.Chromik, R.Kirchhoff, A.Löwen, K.Mosel, K.Plitzko, L.Scholz, L.Wende, M.Wiebel, N.H.Schebb

Structure

Common name

17(RS)-10-epi-SC-D15-11-dihomo-IsoF

LMID

LMFA04020529

Systematic name

(R,Z)-10-hydroxy-10-((2S,4S,5S)-4-hydroxy-5-((E)-3-hydroxyoct-1-en-1-yl)tetrahydrofuran-2-yl)dec-7-enoic acid

m/z

397.3

Adduct / Ion

[M-H]-

Charge

-1

Ion source

ESI

Instrument

Sciex 6500+/QTrap

Resolution

N/A

Ion mode

Negative

Collision Energy (CE)

-20 - -30

Column

Zorbax Eclipse Plus C18 reversed phase column (2.1 x 150 mm, particle size 1.8 mm; Agilent, Waldbronn, Germany)

Mobile phase A

95/5 (v/v) 0.1% acetic acid (aq)/Eluent B

Mobile phase B

800/150/1 (v/v/v) acetonitrile/methanol/acetic acid

Retention time (min)

11.85

Amount injected

~ 0.3 ng

DOI for method

10.1016/j.aca.2017.11.002

Date acquired

February 2025

Submitters

N.Kampschulte, M.A.Chromik, R.Kirchhoff, A.Löwen, K.Mosel, K.Plitzko, L.Scholz, L.Wende, M.Wiebel, N.H.Schebb

Source of standard

chemical synthesis by T. Durand and Coworkers