Common Name
LM ID
Instrument
Adduct / Ion
Submitter
Structure
Common name
22R-hydroxycholesterol
LMID
Systematic name
cholest-5-en-3beta,22R-diol
Adduct / Ion
[M+NH4]+
Charge
1
Ion source
ESI
Instrument
SciEx
Ion mode
Positive
Collision Energy (CE)
10.0V
Source of standard
Cayman Chemical
22R-hydroxycholesterol
Thermo Scientific Orbitrap Exploris 240
[M+H-H2O]+
Fedorova Lab (LMAI) - Gabriele Lombardi Bendoula
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Structure
Common name
22R-hydroxycholesterol
LMID
Systematic name
cholest-5-en-3beta,22R-diol
m/z
385.3465
Adduct / Ion
[M+H-H2O]+
Charge
1
Ion source
HESI
Instrument
Thermo Scientific Orbitrap Exploris 240
Resolution
30000
For high resolution data only
Ion mode
Positive
Collision Energy (CE)
10,20,30
Column
Accucore C30 150 x 2.2
Mobile phase A
Acetonitrile:water 50/50 v/v 5mM AmFm + 0.1% FA
Mobile phase B
Isopropanol:Acetonitrile:water 85/10/5 v/v 5mM AmFm + 0.1% FA
Retention time (min)
6.67
DOI for method
Date acquired
December 2025
Submitters
Fedorova Lab (LMAI) - Gabriele Lombardi Bendoula
Source of standard
Cayman Chemical
22R-hydroxycholesterol
Thermo Scientific Orbitrap Exploris 240
[M+H-H2O]+
Fedorova Lab (LMAI) - Gabriele Lombardi Bendoula
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tensity":6399.7587890625},{"mz":325.2897339,"intensity":8627.197265625},{"mz":331.6955872,"intensity":1729.6444091797},{"mz":367.335907,"intensity":279380.65625},{"mz":385.3465576,"intensity":184071.78125}]
Structure
Common name
22R-hydroxycholesterol
LMID
Systematic name
cholest-5-en-3beta,22R-diol
m/z
385.3465
Adduct / Ion
[M+H-H2O]+
Charge
1
Ion source
HESI
Instrument
Thermo Scientific Orbitrap Exploris 240
Resolution
30000
For high resolution data only
Ion mode
Positive
Collision Energy (CE)
20,30,40
Column
Accucore C30 150 x 2.2
Mobile phase A
Acetonitrile:water 50/50 v/v 5mM AmFm + 0.1% FA
Mobile phase B
Isopropanol:Acetonitrile:water 85/10/5 v/v 5mM AmFm + 0.1% FA
Retention time (min)
6.67
DOI for method
Date acquired
December 2025
Submitters
Fedorova Lab (LMAI) - Gabriele Lombardi Bendoula
Source of standard
Cayman Chemical
22R-hydroxycholesterol
Thermo Scientific Orbitrap Exploris 240
[M+H-H2O]+
Fedorova Lab (LMAI) - Gabriele Lombardi Bendoula
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Structure
Common name
22R-hydroxycholesterol
LMID
Systematic name
cholest-5-en-3beta,22R-diol
m/z
385.3465
Adduct / Ion
[M+H-H2O]+
Charge
1
Ion source
HESI
Instrument
Thermo Scientific Orbitrap Exploris 240
Resolution
30000
For high resolution data only
Ion mode
Positive
Collision Energy (CE)
30,40,50
Column
Accucore C30 150 x 2.2
Mobile phase A
Acetonitrile:water 50/50 v/v 5mM AmFm + 0.1% FA
Mobile phase B
Isopropanol:Acetonitrile:water 85/10/5 v/v 5mM AmFm + 0.1% FA
Retention time (min)
6.67
DOI for method
Date acquired
December 2025
Submitters
Fedorova Lab (LMAI) - Gabriele Lombardi Bendoula
Source of standard
Cayman Chemical