Lipidomics studies on macrophages - bone marrow-derived macrophages treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

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Details:

Legend:graph_icon: View detailed data for replicatesgraph_icon: View time course graphs
Ctrl:Untreated BMDM cells (control)
25-OHC: Cells treated with 25-OH-cholesterol at time 0
*: BMDM cells in lipid-depleted media containing compactin
25-OHC*: cells in lipid-depleted media containing compactin and treated with 25-OHC at time 0
PA| PC| PE| PG| PI| PS| DNA| TNF

Details Graph
Individual
Expts		Ave Biol. Reps
LM_ID Name 25-OHC*
*
25-OHC
Ctrl
 0 hrs		 25-OHC*
*
25-OHC
Ctrl
 30 min		 25-OHC*
*
25-OHC
Ctrl
 1 hr		 25-OHC*
*
25-OHC
Ctrl
 2 hrs		 25-OHC*
*
25-OHC
Ctrl
 4 hrs		 25-OHC*
*
25-OHC
Ctrl
 8 hrs		 25-OHC*
*
25-OHC
Ctrl
 12 hrs		 25-OHC*
*
25-OHC
Ctrl
 24 hrs		Units
. . . .
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		 PI(34:1) -
22
-
35.6		 22.7
43.8
24.5
18.1		 32.4
26.7
25.2
21		 27
33
25.1
25.2		 36.6
41.5
25.3
20.4		 51.8
44
45.6
30.9		 91.5
45.4
37.4
38.6		 74.5
101
72.8
42.7		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100427)		 PI(34:1) -
25.8
-
28		 26.7
48.2
37.3
17.6		 49.7
60.7
16.8
48.3		 53.4
54.3
81.4
25.7		 49.1
50.8
68.3
61		 52
49.3
92.3
71.3		 79.6
49.2
61.2
53.6		 172
141
118
76.4		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100624)		 PI(34:1) -
45.9
-
54.3		 45
52.6
37.3
44.4		 -
53.4
39.7
81.2		 75.2
-
35.3
68.2		 48.2
94.8
-
49.4		 56.4
88.8
73.9
137		 119
153
155
126		 186
117
76.7
86		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		 PI(34:2) -
15.6
-
17.1		 7.91
15.8
10.5
9.01		 12.6
9.34
9.38
8.25		 9.73
13.5
9.91
10.8		 12.5
14.5
8.61
9.51		 17
17
18
13.8		 19.1
22.4
14.9
16.4		 22.2
29.4
23
17		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100427)		 PI(34:2) -
11.4
-
16.2		 13.4
23.6
20.6
9.49		 20.8
27.6
9.93
22.7		 21.8
22.8
33.7
13.9		 20.5
21.6
27.6
27.8		 17.3
20.8
40.1
37.9		 28.2
22
19.4
21.8		 62.7
70.8
45.3
36		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100624)		 PI(34:2) -
27.3
-
31.4		 27.6
28.5
22.2
26.5		 -
32.7
26.1
46.3		 40.7
-
21.5
34.2		 24.6
44
-
28.9		 24.4
43.5
32.5
59.9		 40.6
60.3
53.1
61.7		 45.6
44.4
18.9
37.8		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		 PI(36:1) -
30.7
-
38.7		 27.9
45.7
24.8
20.2		 57.6
37.9
32.7
24.1		 51.3
47.4
47.3
46.7		 52.5
64.4
44.1
29.4		 76.9
63.8
54.9
43.7		 108
130
46.5
56.6		 105
133
75.3
52.9		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100427)		 PI(36:1) -
41.2
-
34.1		 46.7
76.7
62.8
25.7		 75.4
102
28.6
79.3		 82.8
77.5
116
41.2		 72.1
73.2
93.8
90.4		 68.2
62.4
106
97.4		 102
62
62
60.2		 208
189
119
91.5		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100624)		 PI(36:1) -
56.5
-
55.6		 58.5
65.3
44.3
51.5		 -
56.4
43.7
97.7		 107
-
48.7
98.3		 63.5
135
-
60.8		 70.5
131
86.3
121		 118
141
124
99.8		 165
112
57.1
83.5		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		 PI(36:2) -
52.7
-
92.5		 57
98.3
74.3
57.5		 112
84.4
95.6
61.2		 93
62.5
106
129		 64.6
83.3
69.3
44.5		 85.1
79.9
80.4
64		 227
240
63.8
81.6		 219
301
192
172		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100427)		 PI(36:2) -
71.1
-
64.3		 97.1
162
130
50.9		 141
182
63.1
172		 143
148
238
92.5		 132
141
184
175		 129
125
206
196		 166
131
125
130		 348
351
222
192		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100624)		 PI(36:2) -
124
-
136		 148
156
130
126		 -
162
137
254		 156
-
78.6
183		 87.1
188
-
97.9		 96.6
211
123
330		 229
329
277
289		 290
233
118
189		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		 PI(36:3) -
13
-
28.9		 11.4
21.7
20.2
15.7		 25.4
17.2
27.4
16		 20.2
17.2
26.4
26.9		 15.4
21.2
20.1
14.5		 20.8
20.8
28.2
21.5		 36.5
50.2
22.8
23.9		 36.8
47.9
56.7
39		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100427)		 PI(36:3) -
15.8
-
18.3		 19.2
33.2
36.8
14.6		 29.5
41.9
17.2
45		 29.5
32.8
67.8
21.9		 28.7
30.4
52.4
42.5		 26.7
26.8
51.6
50.8		 32.7
25.4
28.9
27.6		 65.6
72.8
51
42.7		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100624)		 PI(36:3) -
26
-
33.4		 28
32.3
29.7
29.6		 -
37.1
33.6
58.9		 38.7
-
23.8
43		 20.8
45.1
-
28		 21.9
47.5
38.1
76.6		 42.4
61
61.9
54.5		 59.7
45.4
26.2
35.2		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		 PI(36:4) -
15.7
-
29		 12.4
24.5
18.3
16.6		 22.9
18.5
22.9
16.4		 18.1
15
26.4
29.6		 14.7
18.5
20
13.4		 19.6
18.1
28.4
18.9		 42.8
39.2
22.7
22.1		 28.8
43.9
66
40.1		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100427)		 PI(36:4) -
15.5
-
22.1		 19.1
33.9
34.8
15.7		 28.2
39.1
17.4
42.5		 26.2
30.7
69.1
21.4		 27.3
29.3
53.5
44.8		 27.1
25.9
67.8
53.9		 35.8
23.6
47.5
32.4		 68.8
59.9
71
43.1		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100624)		 PI(36:4) -
22
-
32.8		 22.3
26
25.9
27.7		 -
36.3
30.6
54.6		 29.7
-
20.1
40.2		 17.2
35
-
22.9		 20.6
40.5
35.3
74.8		 45.6
60.8
86.7
68.7		 74.2
53.5
39.3
42		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		 PI(38:2) -
17.2
-
30.5		 12.4
17.8
18.1
18.3		 17.7
16.6
19.6
14.5		 18.2
27.1
21.7
29.6		 28.1
30
30.4
21.2		 37.8
34.8
47.3
35		 34.3
38.4
37
42.5		 29.2
43.1
33.2
33.6		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100427)		 PI(38:2) -
18.3
-
17.5		 20.3
37.1
38.8
14.4		 33.6
46.1
17.5
45		 34
32.2
65.5
21.8		 30
31.3
50.7
47.5		 24.4
24.4
42.4
42.7		 32.2
22.8
26.2
25		 75.7
70.6
46.5
35.6		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100624)		 PI(38:2) -
19.1
-
26.6		 20.1
22.1
18.5
19.4		 -
27.1
22
39.6		 47
-
29
44.8		 32.4
60.5
-
32		 32.4
63.7
46.3
41.1		 37.2
48.8
38.7
39.6		 51.9
36.4
18.1
27.8		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		 PI(38:3) -
69.9
-
127		 50.8
80.8
92.8
73.6		 81.7
63.2
101
82.9		 70.4
90.1
115
136		 87.6
101
113
78.7		 117
109
173
130		 117
154
136
148		 90
171
150
145		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100427)		 PI(38:3) -
62.2
-
66.6		 80.7
144
172
56.8		 125
179
74.9
200		 126
132
300
93.9		 110
125
215
193		 95.7
103
196
185		 131
91.7
112
110		 256
265
170
150		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100624)		 PI(38:3) -
84
-
126		 87
97.6
94.3
98.1		 -
98.7
103
197		 182
-
131
200		 114
244
-
143		 117
229
200
216		 138
174
189
185		 181
127
75.9
107		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		 PI(38:4) -
110
-
200		 96
147
159
140		 149
118
196
138		 126
140
188
237		 134
161
167
122		 175
169
214
170		 217
329
173
202		 196
300
337
282		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100427)		 PI(38:4) -
113
-
125		 141
249
296
104		 205
289
140
340		 202
215
498
167		 183
207
358
326		 176
190
368
335		 210
168
221
203		 419
424
313
268		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100624)		 PI(38:4) -
148
-
228		 155
172
187
182		 -
194
221
357		 260
-
180
351		 147
328
-
197		 161
342
271
399		 263
317
391
337		 356
229
164
199		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		 PI(38:5) -
40
-
101		 42.8
75
66.9
58.5		 83
64
94.1
59.1		 63
65.7
103
121		 58.7
78.4
82.3
56.7		 76.6
75.7
90.2
72		 113
151
71.3
84.6		 107
147
142
132		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100427)		 PI(38:5) -
60.5
-
68.4		 88.8
150
161
55.1		 120
168
80.2
194		 114
129
275
102		 97.5
122
202
183		 87.9
91
189
198		 101
86.6
119
113		 169
192
159
156		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100624)		 PI(38:5) -
123
-
178		 124
132
149
149		 -
134
158
252		 188
-
130
262		 97.4
204
-
127		 113
240
181
266		 150
195
240
234		 176
139
102
160		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		 PI(38:6) -
6.65
-
10.2		 5.08
5.61
5.39
4.8		 10.3
4.26
10
5.79		 6.33
7.91
9.48
9.55		 7.17
8.9
9.38
6.97		 8.16
8.92
10.2
8.63		 9.98
18.8
8.67
10.2		 12.1
24.1
26.9
11.6		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100427)		 PI(38:6) -
5.71
-
9.31		 6.61
10.6
11.9
5.89		 8.8
11.6
6.55
15.8		 7.84
9.16
20.2
8.01		 7.29
7.73
14.5
12.5		 7.94
5.88
11.6
13.9		 7.42
6.96
8.47
7.62		 13.2
15
10.5
9.85		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100624)		 PI(38:6) -
7.93
-
11.6		 9.44
8.44
8.05
9.03		 -
19.6
12.4
19.7		 16.6
-
9
14.4		 8.76
17.2
-
10.9		 9.86
20.4
14.4
22.7		 13.2
19.2
18.6
23.4		 17.1
17.8
7.31
10.4		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		 PI(40:4) -
7.73
-
11.3		 3.84
6.65
6.96
6.07		 6.13
3.55
6.29
8.25		 6.09
7.82
7.76
11.1		 7.89
8.4
10.4
7.7		 9.79
8.81
15
11.4		 11.9
15.3
12.2
13.8		 18.9
16.4
18.5
12.1		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100427)		 PI(40:4) -
5.71
-
8.27		 7.19
12.3
14.7
6.11		 9.91
15.7
7.45
17.5		 9.3
10.6
27.8
7.9		 9.3
9.38
19.4
17		 5.83
7.48
15.2
15.2		 9.11
6.57
10.7
9.56		 16.7
17.3
14.4
10.2		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100624)		 PI(40:4) -
10.5
-
15.1		 10.3
10.3
10.2
11		 -
16.4
13.9
21.5		 17.8
-
12.6
22		 10.7
21.7
-
13.4		 10.9
26.8
18.4
26.2		 16.6
21.8
18.6
25.4		 19.6
19.1
8.98
10.8		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		 PI(40:5) -
8.81
-
18.4		 6.44
8.73
12
9.21		 9.81
10.1
12.2
9.81		 6.72
11.9
15.2
14.7		 11.9
13.8
15.8
11.4		 15.9
15.5
25.6
19.1		 19.1
19.9
20.4
22.8		 20.3
24.4
32.7
21.3		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100427)		 PI(40:5) -
9.49
-
13		 11.7
21.6
29.9
10		 17.4
24.9
12.5
29.9		 18
17.1
42.4
15.3		 14.7
17.2
31.8
29.3		 13
11.8
27.5
28.5		 15
11.4
14.4
15.8		 29.7
30.9
26
22.7		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100624)		 PI(40:5) -
14.2
-
21.6		 14.1
16.4
16.4
17		 -
26.5
18.5
31.6		 29.7
-
19.9
34		 16.8
35.6
-
22.7		 17.4
40.5
33.2
41.5		 22.3
31
34.2
36.9		 30.5
23.9
14.6
20.5		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		 PI(40:6) -
5.87
-
13.9		 4.63
6.75
7.64
6.47		 12.9
8.16
10.7
9.03		 8.07
10.7
11.6
12.7		 9.69
11.3
13
9.33		 13.3
13.1
21.8
16.7		 13.8
27.2
17.9
18.5		 19
18.9
25.1
16.4		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100427)		 PI(40:6) -
7.49
-
11		 9.5
18.2
21.7
6.98		 13
19.4
10.3
23.9		 13
13.9
32.8
11.4		 10
11.8
23.5
22.4		 8.04
8.12
19.3
20		 10.3
9.28
11.1
10.4		 19.2
20.2
18
15.5		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100624)		 PI(40:6) -
13.1
-
18.2		 12.2
13.4
14.2
13.9		 -
25.1
16.9
29.3		 23.7
-
17.4
28.2		 15.2
29.1
-
19.5		 15.7
36.9
27.4
34.5		 19.9
30.5
29.1
32		 24.8
23.5
12.5
16.2		 pmol/sample