Lipidomics studies on macrophages - Foam cell study with wild-type and LDLR(-/-) mice, normal and high-fat diets

*: In HPLC/MS/MS measurements by multiple reaction monitoring, the response ratio for each analyte was determined relative to a known amount of internal standard (400 pmol of 13C18 (18:1) cholesterol ester) that was added to each biological and calibration sample. The absolute amount of each analyte in a sample was then calculated by comparison to a standard curve for a given analyte. Specific six point calibration curves from 0.0 to 1.6 nmoles were constructed for each set of samples for the following cholesterol esters: 14:0, 16:0, 16:1, 18:0, 18:1, 18:2, 20:0, 20:1, 20:2, 20:3, 20:4, and 22:6. Analytes for which no direct reference standard was available were determined based on the curve for a standard closest in number of carbon atoms and double bonds. The value reported in the table was finally adjusted for the amount of DNA measured for each sample.