Lipidomics studies on macrophages - RAW 264.7 cells treated with Kdo2-Lipid A

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Acyl CoA's (FA)

Display Time Course Plots for Selected Analytes

Legendgraph_icon: View data/graphs for technical replicates
graph icon: Graph of biological replicates(across expts.)
Details/Graph
 Name Kdo
Ctl
 0min		 Kdo
Ctl
 30min		 Kdo
Ctl
 1hr		 Kdo
Ctl
 2hrs		 Kdo
Ctl
 4hrs		 Kdo
Ctl
 8hrs		 Kdo
Ctl
 12hrs		 Kdo
Ctl
 24hrs
Units
. . . .
 details icon   graph iconCoA(14:0)-
0.74		 0.70
0.82		 0.55
0.65		 0.47
0.64		 0.45
0.54		 0.56
0.58		 0.58
0.60		 0.89
0.80		 pmol/ug DNA
 details icon   graph iconCoA(16:0)-
0.90		 0.84
0.95		 0.62
0.80		 0.60
0.71		 0.65
0.72		 0.81
0.71		 0.99
0.84		 1.59
0.85		 pmol/ug DNA
 details icon   graph iconCoA(16:1)-
0.64		 0.58
0.80		 0.42
0.59		 0.43
0.66		 0.44
0.65		 0.37
0.72		 0.25
0.87		 0.22
1.00		 pmol/ug DNA
 details icon   graph iconCoA(18:0)-
0.32		 0.48
0.39		 0.37
0.30		 0.33
0.28		 0.33
0.26		 0.38
0.26		 0.43
0.33		 0.71
0.45		 pmol/ug DNA
 details icon   graph iconCoA(18:1)-
1.24		 1.33
1.66		 0.93
1.26		 0.90
1.31		 0.91
1.30		 0.74
1.29		 0.74
1.64		 1.10
2.54		 pmol/ug DNA
 details icon   graph iconCoA(18:2)-
0.22		 0.25
0.33		 0.25
0.27		 0.24
0.31		 0.21
0.33		 0.17
0.32		 0.20
0.36		 0.25
0.50		 pmol/ug DNA
 details icon   graph iconCoA(20:0)-
0.02		 0.03
0.03		 0.02
0.02		 0.02
0.02		 0.02
0.02		 0.03
0.02		 0.03
0.03		 0.04
0.05		 pmol/ug DNA
 details icon   graph iconCoA(20:4)-
0.44		 0.58
0.65		 0.52
0.54		 0.45
0.49		 0.39
0.51		 0.37
0.51		 0.47
0.58		 0.51
0.65		 pmol/ug DNA
 details icon   graph iconCoA(22:0)-
0.04		 0.04
0.05		 0.03
0.04		 0.03
0.04		 0.03
0.03		 0.03
0.03		 0.02
0.04		 0.04
0.06		 pmol/ug DNA
 details icon   graph iconCoA(22:6)-
0.04		 0.09
0.10		 0.04
0.04		 0.03
0.04		 0.03
0.04		 0.04
0.03		 0.07
0.04		 0.07
0.04		 pmol/ug DNA
 details icon   graph iconCoA(24:0)-
0.15		 0.14
0.23		 0.10
0.14		 0.08
0.11		 0.09
0.10		 0.08
0.11		 0.10
0.14		 0.29
0.22		 pmol/ug DNA
 details icon   graph iconCoA(24:1)-
0.06		 0.05
0.12		 0.04
0.07		 0.03
0.06		 0.03
0.05		 0.03
0.07		 0.05
0.10		 0.08
0.21		 pmol/ug DNA
 details icon   graph iconCoA(26:0)-
0.07		 0.06
0.07		 0.04
0.06		 0.03
0.06		 0.04
0.06		 0.06
0.07		 0.10
0.13		 0.31
0.16		 pmol/ug DNA
 details icon   graph iconCoA(26:1)-
0.06		 0.03
0.08		 0.04
0.06		 0.03
0.07		 0.04
0.06		 0.03
0.08		 0.05
0.11		 0.10
0.15		 pmol/ug DNA
 details icon   graph iconDNA-
5.87		 4.94
5.29		 6.42
6.30		 7.30
7.03		 8.30
8.19		 8.38
9.22		 9.13
10.34		 8.18
14.47		 ug/sample
 details icon   graph iconTNF-
104.83		 137.33
92.67		 2246.17
110.17		 6675.17
117.67		 20271.33
150.67		 41672.50
198.50		 46510.83
289.67		 50027.50
416.50		 pg/ml


 

*:The units in the table and graphs correspond to the intensity of each species divided by the intensity of an appropriate internal standard (odd-chain length fatty acyl-CoA's as described in Haynes CA, Allegood JC, Sims K, Wang EW, Sullards MC, Merrill AH Jr., J Lipid Res. 49:1113-25, 2008). The amount of internal standard added to each sample in the time series and control experiments was identical. The relative intensity values were converted to pmoles and then normalized to the measured quantity of DNA present in each RAW cell preparation following treatment with and without Kdo2 lipid A for each time point.