Lipidomics studies on macrophages - RAW 264.7 cells treated with Kdo2-Lipid A

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Glycerophospholipids (GP)

  PA | PC | PE | PG | PI | PS | DNA

Display Time Course Plots for Selected Analytes

Legendgraph_icon: View data/graphs for technical replicates
graph icon: Graph of biological replicates(across expts.)
Details/Graph
 Name Kdo
Ctl
 0min		 Kdo
Ctl
 30min		 Kdo
Ctl
 1hr		 Kdo
Ctl
 2hrs		 Kdo
Ctl
 4hrs		 Kdo
Ctl
 8hrs		 Kdo
Ctl
 12hrs		 Kdo
Ctl
 24hrs
Units
. . . .
 details icon   graph iconPA(32:0)4.23
4.42		 4.67
4.13		 4.40
3.57		 5.53
4.45		 6.93
4.40		 15.85
4.63		 55.33
9.37		 70.66
5.10		 pmol/ug DNA
 details icon   graph iconPA(34:0)3.45
3.00		 3.72
3.17		 3.55
3.12		 4.00
3.47		 4.43
3.43		 9.28
3.50		 23.07
6.83		 29.76
2.90		 pmol/ug DNA
 details icon   graph iconPA(34:1)7.65
7.77		 8.38
7.48		 7.77
6.92		 9.38
8.45		 9.23
7.78		 12.48
7.20		 21.78
12.90		 14.30
5.08		 pmol/ug DNA
 details icon   graph iconPA(34:2)1.40
1.33		 1.28
1.13		 1.22
1.15		 1.43
1.32		 1.63
1.35		 1.43
1.10		 1.67
2.27		 0.92
0.68		 pmol/ug DNA
 details icon   graph iconPA(36:0)3.25
2.03		 2.78
2.18		 2.30
1.72		 2.75
2.47		 2.30
1.88		 4.40
1.90		 9.53
3.55		 13.07
1.88		 pmol/ug DNA
 details icon   graph iconPA(36:1)5.72
5.84		 7.12
5.17		 6.53
4.77		 7.40
5.65		 6.95
5.48		 9.27
5.87		 17.85
8.08		 16.66
6.26		 pmol/ug DNA
 details icon   graph iconPA(36:2)4.47
4.86		 4.25
3.55		 4.65
3.90		 5.52
4.55		 4.18
3.88		 5.02
3.57		 7.27
7.17		 4.58
2.86		 pmol/ug DNA
 details icon   graph iconPA(36:3)0.90
0.95		 1.07
0.75		 1.00
0.62		 1.00
0.72		 0.87
0.72		 0.92
0.55		 1.10
1.15		 0.62
0.40		 pmol/ug DNA
 details icon   graph iconPA(36:4)0.47
0.53		 0.50
0.42		 0.68
0.37		 0.62
0.38		 0.52
0.40		 0.48
0.25		 0.65
0.75		 0.36
0.20		 pmol/ug DNA
 details icon   graph iconPA(38:1)1.12
0.83		 0.90
0.72		 0.62
0.55		 0.87
0.65		 0.65
0.55		 1.22
0.60		 3.07
1.12		 2.70
0.54		 pmol/ug DNA
 details icon   graph iconPA(38:2)1.20
1.38		 1.45
1.15		 1.45
1.23		 1.72
1.32		 1.30
1.12		 2.00
1.03		 3.42
2.00		 2.40
1.14		 pmol/ug DNA
 details icon   graph iconPA(38:3)2.97
2.35		 2.73
2.17		 2.95
2.27		 2.98
2.50		 2.02
1.88		 2.93
1.67		 4.33
3.03		 2.56
1.82		 pmol/ug DNA
 details icon   graph iconPA(38:4)3.55
2.55		 2.77
2.20		 3.33
2.55		 3.00
2.43		 1.75
2.00		 2.33
1.48		 3.22
2.38		 1.88
1.18		 pmol/ug DNA


 

*:The units in the table and graphs correspond to the intensity of each species divided by the average intensity (peak area) of a set of odd-chain internal standards corresponding to that particular headgroup. No internal standards were available for the PI series so intensity of each species was divided by the the total PI intensity in this case. The amount of internal standard added to each sample in the time series and control experiments was identical. The relative intensity values were then normalized to the measured quantity of DNA present in each RAW cell preparation following treatment with and without Kdo2 lipid A for each time point.