Lipidomics studies on macrophages - RAW 264.7 cells treated with Kdo2-Lipid A

Introduction | Browse | Search | Download
Mouse-over the above links for more information

Glycerolipids (GL)

  Diacylglycerols | Triacylglycerols | DNA | TNF

Display Time Course Plots for Selected Analytes

Legendgraph_icon: View data/graphs for technical replicates
graph icon: Graph of biological replicates(across expts.)
Details/Graph
 Name Kdo
Ctl
 0min		 Kdo
Ctl
 30min		 Kdo
Ctl
 1hr		 Kdo
Ctl
 2hrs		 Kdo
Ctl
 4hrs		 Kdo
Ctl
 8hrs		 Kdo
Ctl
 12hrs		 Kdo
Ctl
 24hrs
Units
. . . .
 details icon   graph iconDG(14:0/16:0)_14:0-
0.02		 0.03
0.02		 0.04
0.03		 0.03
0.02		 0.03
0.03		 0.04
0.02		 0.05
0.03		 0.05
0.02		 Ratio Int/ug DNA
 details icon   graph iconDG(14:0/16:0)_16:0-
0.02		 0.03
0.02		 0.04
0.03		 0.03
0.02		 0.03
0.03		 0.04
0.02		 0.06
0.03		 0.05
0.02		 Ratio Int/ug DNA
 details icon   graph iconDG(14:0/16:1)_14:0-
0.01		 0.01
0.01		 0.02
0.01		 0.01
0.01		 0.01
0.01		 0.01
0.01		 0.02
0.01		 0.02
0.01		 Ratio Int/ug DNA
 details icon   graph iconDG(14:0/18:1)_14:0-
0.03		 0.05
0.04		 0.07
0.05		 0.04
0.04		 0.03
0.05		 0.03
0.03		 0.05
0.04		 0.04
0.03		 Ratio Int/ug DNA
 details icon   graph iconDG(14:1/16:0)_16:0-
0.01		 0.01
0.01		 0.01
0.01		 0.01
0.01		 0.01
0.01		 0.01
0.01		 0.01
0.01		 0.01
0.00		 Ratio Int/ug DNA
 details icon   graph iconDG(15:0/16:0)_15:0-
0.04		 0.05
0.05		 0.05
0.05		 0.04
0.03		 0.05
0.06		 0.08
0.07		 0.14
0.10		 0.21
0.05		 Ratio Int/ug DNA
 details icon   graph iconDG(15:0/16:1)_15:0-
0.02		 0.02
0.02		 0.03
0.03		 0.02
0.02		 0.02
0.03		 0.03
0.03		 0.04
0.04		 0.06
0.02		 Ratio Int/ug DNA
 details icon   graph iconDG(15:0/18:1)_15:0-
0.05		 0.06
0.06		 0.07
0.07		 0.06
0.05		 0.07
0.09		 0.09
0.09		 0.16
0.12		 0.22
0.06		 Ratio Int/ug DNA
 details icon   graph iconDG(16:0/16:0)_16:0-
0.13		 0.15
0.13		 0.23
0.18		 0.16
0.12		 0.21
0.19		 0.27
0.13		 0.48
0.16		 0.46
0.08		 Ratio Int/ug DNA
 details icon   graph iconDG(16:0/17:0)_17:0-
0.07		 0.08
0.08		 0.09
0.09		 0.06
0.06		 0.09
0.09		 0.10
0.10		 0.15
0.15		 0.17
0.10		 Ratio Int/ug DNA
 details icon   graph iconDG(16:0/17:1)_16:0-
0.01		 0.01
0.01		 0.01
0.01		 0.01
0.01		 0.01
0.01		 0.01
0.01		 0.02
0.01		 0.01
0.00		 Ratio Int/ug DNA
 details icon   graph iconDG(16:0/18:0)_16:0-
0.04		 0.04
0.04		 0.06
0.05		 0.04
0.03		 0.06
0.04		 0.08
0.04		 0.13
0.06		 0.10
0.02		 Ratio Int/ug DNA
 details icon   graph iconDG(16:0/18:1)_16:0-
0.23		 0.32
0.26		 0.49
0.45		 0.27
0.28		 0.27
0.40		 0.37
0.30		 0.56
0.36		 0.43
0.21		 Ratio Int/ug DNA
 details icon   graph iconDG(16:0/18:2)_16:0-
0.01		 0.02
0.02		 0.03
0.03		 0.02
0.02		 0.02
0.02		 0.02
0.01		 0.03
0.02		 0.02
0.01		 Ratio Int/ug DNA
 details icon   graph iconDG(16:0/19:0)_16:0-
0.02		 0.02
0.01		 0.02
0.02		 0.01
0.01		 0.01
0.01		 0.01
0.01		 0.01
0.00		 0.01
0.01		 Ratio Int/ug DNA
 details icon   graph iconDG(16:0/19:0)_19:0-
0.03		 0.04
0.03		 0.03
0.05		 0.03
0.02		 0.05
0.04		 0.04
0.04		 0.04
0.03		 0.04
0.02		 Ratio Int/ug DNA
 details icon   graph iconDG(16:0/20:1)_16:0-
0.03		 0.03
0.03		 0.05
0.04		 0.02
0.03		 0.03
0.04		 0.03
0.03		 0.04
0.04		 0.03
0.02		 Ratio Int/ug DNA
 details icon   graph iconDG(16:0/20:2)_16:0-
0.02		 0.02
0.03		 0.04
0.05		 0.02
0.02		 0.01
0.02		 0.02
0.02		 0.02
0.02		 0.02
0.01		 Ratio Int/ug DNA
 details icon   graph iconDG(16:0/20:3)_16:0-
0.00		 0.01
0.01		 0.02
0.01		 0.01
0.01		 0.01
0.01		 0.01
0.00		 0.01
0.01		 0.01
0.00		 Ratio Int/ug DNA
 details icon   graph iconDG(16:0/20:4)_16:0-
0.01		 0.01
0.01		 0.01
0.01		 0.01
0.00		 0.01
0.01		 0.00
0.00		 0.01
0.00		 0.01
0.00		 Ratio Int/ug DNA
 details icon   graph iconDG(16:0/20:4)_20:4-
0.01		 0.01
0.01		 0.02
0.01		 0.01
0.00		 0.01
0.01		 0.01
0.00		 0.01
0.01		 0.01
0.00		 Ratio Int/ug DNA
 details icon   graph iconDG(16:1/16:0)_16:0-
0.08		 0.12
0.09		 0.19
0.16		 0.11
0.10		 0.11
0.15		 0.14
0.10		 0.23
0.11		 0.18
0.06		 Ratio Int/ug DNA
 details icon   graph iconDG(16:1/20:4)_20:4-
0.00		 0.00
0.00		 0.01
0.00		 0.00
0.00		 0.00
0.00		 0.00
0.00		 0.00
0.00		 0.00
0.00		 Ratio Int/ug DNA
 details icon   graph iconDG(17:0/18:1)_17:0-
0.03		 0.04
0.04		 0.06
0.07		 0.04
0.04		 0.04
0.07		 0.06
0.05		 0.08
0.08		 0.07
0.06		 Ratio Int/ug DNA
 details icon   graph iconDG(18:0/20:4)_20:4-
0.01		 0.02
0.02		 0.03
0.02		 0.01
0.01		 0.01
0.02		 0.01
0.01		 0.01
0.01		 0.01
0.01		 Ratio Int/ug DNA
 details icon   graph iconDG(18:1/20:4)_20:4-
0.01		 0.01
0.01		 0.03
0.01		 0.01
0.01		 0.01
0.01		 0.01
0.01		 0.01
0.01		 0.00
0.01		 Ratio Int/ug DNA
 details icon   graph iconDG(20:0/20:0)_20:0-
0.05		 0.06
0.04		 0.05
0.11		 0.07
0.05		 0.23
0.07		 0.05
0.04		 0.05
0.05		 0.04
0.03		 Ratio Int/ug DNA
 details icon   graph iconDG(20:4/20:0)_20:4-
0.01		 0.01
0.01		 0.02
0.01		 0.01
0.01		 0.01
0.01		 0.01
0.01		 0.01
0.01		 0.00
0.01		 Ratio Int/ug DNA


 

*:The units in the table and graphs correspond to the abundance of each [M+NH4]+ ion measured as a neutral loss for a specific fatty acid (+NH3) divided by the signal from the d5-labeled internal standard containing that specific fatty acid, obtained in the same neutral loss scan. The amount of internal standard added to each sample in the time series and control experiments was identical. The ratio of these ion abundances (Ratio Int.) is then normalized to the measured quantity of DNA present in each RAW cell preparation following treatment with and without Kdo2 lipid A for each time point.