Lipidomics studies on macrophages - RAW 264.7 cells treated with Kdo2-Lipid A

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Eicosanoids (FA) [media]

Display Time Course Plots for Selected Analytes

Legendgraph_icon: View data/graphs for technical replicates
graph icon: Graph of biological replicates(across expts.)
Details/Graph
 Name Kdo
Ctl
 0min		 Kdo
Ctl
 30min		 Kdo
Ctl
 1hr		 Kdo
Ctl
 2hrs		 Kdo
Ctl
 4hrs		 Kdo
Ctl
 8hrs		 Kdo
Ctl
 12hrs		 Kdo
Ctl
 24hrs
Units
. . . .
 details icon   graph icon11-HETE-
0.47		 0.56
0.41		 0.81
0.41		 1.02
0.38		 3.07
0.30		 6.99
0.19		 4.74
0.10		 1.36
0.02		 pmol/ug DNA
 details icon   graph icon15-deoxy-PGD2-
0.22		 0.23
0.20		 0.37
0.15		 0.48
0.16		 2.36
0.17		 18.26
0.15		 37.23
0.17		 72.66
0.11		 pmol/ug DNA
 details icon   graph icon15-deoxy-PGJ2-
0.16		 0.12
0.11		 0.12
0.10		 0.13
0.11		 0.26
0.10		 2.03
0.08		 7.16
0.07		 23.49
0.05		 pmol/ug DNA
 details icon   graph icon5-HETE-
0.11		 0.10
0.09		 0.12
0.10		 0.10
0.09		 0.07
0.07		 0.04
0.04		 0.03
0.02		 0.02
0.01		 pmol/ug DNA
 details icon   graph iconArachidonic acid-
80.72		 147.16
73.42		 356.58
72.93		 318.89
68.77		 214.36
49.02		 99.18
27.01		 39.43
14.08		 10.49
3.89		 pmol/ug DNA
 details icon   graph iconPGD2-
0.70		 4.43
0.61		 8.98
0.65		 14.08
0.79		 70.30
0.59		 193.12
0.48		 159.73
0.38		 82.17
0.17		 pmol/ug DNA
 details icon   graph iconPGE2-
0.17		 0.34
0.22		 0.67
0.23		 0.99
0.25		 3.01
0.24		 12.95
0.16		 17.91
0.14		 22.60
0.08		 pmol/ug DNA
 details icon   graph iconPGF2a-
0.82		 0.90
0.70		 0.81
0.85		 1.03
0.79		 1.59
0.75		 4.05
0.58		 4.54
0.38		 6.89
0.16		 pmol/ug DNA
 details icon   graph iconPGJ2-
0.49		 0.49
0.42		 0.81
0.43		 1.23
0.51		 5.22
0.56		 37.10
0.49		 66.17
0.47		 109.06
0.33		 pmol/ug DNA
 details icon   graph iconDNA-
1.22		 1.35
1.35		 1.33
1.42		 1.58
1.44		 1.65
1.60		 1.81
2.14		 2.13
3.00		 2.15
6.21		 ug/sample
 details icon   graph iconTNF-
39.14		 65.19
45.43		 841.98
49.32		 3205.43
71.98		 12128.77
91.42		 22550.49
97.04		 28015.12
142.47		 36130.19
251.54		 pg/ml


 

*:The units in the table and graphs correspond to the intensity of each analyte divided by the intensity of the corresponding deuterated internal standard. The amount of internal standard added to each sample in the time series and control experiments was identical. The relative intensity values were converted to pmoles by extrapolating from the standard curve for that analyte and then normalized to the number of cells in a given sample as measured by the quantity of DNA in the well.