Lipidomics studies on macrophages - RAW 264.7 cells treated with Kdo2-Lipid A

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Sterol Lipids (ST) - Cholesteryl esters

Display Time Course Plots for Selected Analytes

Legendgraph_icon: View data/graphs for technical replicates
graph icon: Graph of biological replicates(across expts.)
Details/Graph
 Name Kdo
Ctl
 0min		 Kdo
Ctl
 30min		 Kdo
Ctl
 1hr		 Kdo
Ctl
 2hrs		 Kdo
Ctl
 4hrs		 Kdo
Ctl
 8hrs		 Kdo
Ctl
 12hrs		 Kdo
Ctl
 24hrs
Units
. . . .
 details icon   graph iconCE(14:0)-
6.76		 6.41
7.03		 6.20
6.21		 7.05
7.72		 11.54
8.61		 39.52
9.54		 43.28
13.51		 142.10
24.39		 pmol/ug DNA
 details icon   graph iconCE(14:1)-
0.70		 0.59
0.63		 0.58
0.60		 0.73
1.02		 1.07
1.12		 3.96
1.20		 3.10
2.07		 7.04
1.73		 pmol/ug DNA
 details icon   graph iconCE(15:0)-
1.19		 1.07
1.12		 1.10
1.08		 1.13
1.19		 1.74
1.21		 3.81
1.22		 4.29
1.56		 11.71
2.31		 pmol/ug DNA
 details icon   graph iconCE(15:1)-
0.35		 0.00
0.00		 0.00
0.00		 0.25
0.49		 0.31
0.31		 0.85
0.30		 0.60
0.40		 1.41
0.37		 pmol/ug DNA
 details icon   graph iconCE(16:0)-
18.71		 17.24
18.84		 19.01
17.71		 19.10
19.87		 38.35
18.90		 93.25
20.99		 140.51
31.94		 428.26
58.95		 pmol/ug DNA
 details icon   graph iconCE(16:1)-
9.78		 8.21
9.91		 9.13
9.58		 10.95
14.20		 15.20
16.12		 51.47
17.52		 44.60
24.47		 112.65
25.68		 pmol/ug DNA
 details icon   graph iconCE(16:2)-
0.48		 0.39
0.47		 0.45
0.47		 0.53
0.77		 0.64
0.77		 1.91
0.83		 1.44
1.30		 2.46
1.04		 pmol/ug DNA
 details icon   graph iconCE(17:0)-
1.66		 1.59
1.53		 1.68
1.49		 1.54
1.57		 2.50
1.54		 4.43
1.38		 5.91
2.08		 17.70
3.31		 pmol/ug DNA
 details icon   graph iconCE(17:1)-
1.70		 1.48
1.78		 1.71
1.73		 1.84
2.13		 2.40
2.34		 6.15
2.14		 5.68
2.56		 12.82
2.90		 pmol/ug DNA
 details icon   graph iconCE(18:0)-
4.66		 4.29
3.98		 4.59
4.00		 4.04
4.04		 8.01
3.64		 15.37
3.87		 25.86
6.88		 90.59
18.27		 pmol/ug DNA
 details icon   graph iconCE(18:1)-
43.31		 37.62
43.99		 39.77
42.04		 39.23
44.69		 53.05
42.61		 118.53
48.60		 150.22
64.08		 436.08
125.11		 pmol/ug DNA
 details icon   graph iconCE(18:2)-
6.13		 8.51
6.58		 6.13
11.05		 6.81
9.94		 7.82
10.65		 22.49
9.50		 16.70
13.06		 29.04
12.90		 pmol/ug DNA
 details icon   graph iconCE(18:3)-
0.74		 0.61
0.80		 0.79
0.86		 0.87
1.31		 1.08
1.39		 3.13
1.14		 2.32
1.60		 3.09
1.22		 pmol/ug DNA
 details icon   graph iconCE(20:0)-
0.33		 0.36
0.00		 0.39
0.34		 0.41
0.32		 0.53
0.30		 0.77
0.26		 1.29
0.42		 5.21
1.12		 pmol/ug DNA
 details icon   graph iconCE(20:1)-
2.25		 1.99
1.89		 2.06
1.89		 1.67
1.81		 2.21
1.49		 3.23
1.59		 5.10
2.47		 15.54
7.27		 pmol/ug DNA
 details icon   graph iconCE(20:2)-
1.59		 1.37
1.59		 1.39
1.52		 1.43
1.72		 1.44
1.66		 3.41
1.76		 3.35
2.25		 7.32
4.18		 pmol/ug DNA
 details icon   graph iconCE(20:3)-
2.09		 2.14
2.18		 2.56
2.19		 2.75
3.06		 3.22
2.85		 8.80
2.35		 6.64
3.47		 9.39
3.45		 pmol/ug DNA
 details icon   graph iconCE(20:4)-
2.45		 2.16
2.83		 3.23
2.94		 3.40
4.34		 4.34
4.66		 11.94
3.24		 8.73
3.95		 9.88
2.32		 pmol/ug DNA
 details icon   graph iconCE(22:0)-
0.00		 0.00
0.00		 0.00
0.00		 0.00
0.00		 0.27
0.00		 0.27
0.16		 0.49
0.22		 2.31
0.49		 pmol/ug DNA
 details icon   graph iconCE(22:1)-
0.71		 0.69
0.53		 0.81
0.84		 0.60
0.55		 0.86
0.46		 1.01
0.51		 1.60
0.83		 5.43
2.35		 pmol/ug DNA
 details icon   graph iconCE(22:2)-
0.39		 0.39
0.36		 0.36
0.35		 0.32
0.35		 0.35
0.30		 0.54
0.30		 0.69
0.39		 1.53
0.92		 pmol/ug DNA
 details icon   graph iconCE(22:4)-
0.86		 0.75
0.87		 0.84
0.84		 0.84
1.10		 0.75
0.94		 1.73
0.66		 1.30
0.79		 2.80
0.59		 pmol/ug DNA
 details icon   graph iconCE(22:6)-
0.91		 0.79
0.97		 0.91
1.07		 0.97
1.39		 1.05
1.33		 2.81
0.96		 2.12
1.14		 3.10
0.70		 pmol/ug DNA
 details icon   graph iconDNA-
9.51		 9.20
9.35		 10.75
10.12		 11.72
12.50		 15.84
13.70		 14.88
17.93		 16.70
21.16		 20.64
33.57		 ug/sample
 details icon   graph iconTNF-
35.33		 44.67
38.00		 1638.33
46.67		 3365.33
60.67		 13478.00
60.33		 23189.00
105.00		 29551.33
142.33		 38393.33
227.00		 pg/ml


 

*: In HPLC/MS/MS measurements by multiple reaction monitoring, the response ratio for each analyte was determined relative to a known amount of internal standard (400 pmol of 13C18 (18:1) cholesterol ester) that was added to each biological and calibration sample. The absolute amount of each analyte in a sample was then calculated by comparison to a standard curve for a given analyte. Specific six point calibration curves from 0.0 to 1.6 nmoles were constructed for each set of samples for the following cholesterol esters: 14:0, 16:0, 16:1, 18:0, 18:1, 18:2, 20:0, 20:1, 20:2, 20:3, 20:4, and 22:6. Analytes for which no direct reference standard was available were determined based on the curve for a standard closest in number of carbon atoms and double bonds. The value reported in the table was finally adjusted for the amount of DNA measured for each sample.