Lipidomics studies on macrophages - RAW 264.7 cells treated with Kdo₂-Lipid A

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Display Time Course Plots for Selected Analytes

Legend : Graph of technical replicates(within an expt.) : Graph of biological replicates(across expts.)   (BXY123456): Experiment Sample Barcode

Details/Graph technical/biological replicates	Name	KLA Ctl 0min	KLA Ctl 30min	KLA Ctl 1hr	KLA Ctl 2hrs	KLA Ctl 4hrs	KLA Ctl 8hrs	KLA Ctl 12hrs	KLA Ctl 24hrs	Units* . . . .
(BCG060127)	Arachidonic acid	- 94.59	213.46 91.27	450.22 88.10	435.71 92.78	261.17 61.47	127.01 35.47	50.66 18.42	12.17 4.65	pmol/ug DNA
(BCG060201)	Arachidonic acid	- 62.59	133.50 62.62	255.05 68.18	256.49 59.32	222.65 48.77	105.75 27.86	45.95 14.33	10.71 3.84	pmol/ug DNA
(BCG060210)	Arachidonic acid	- 84.98	94.51 66.36	364.46 62.51	264.47 54.20	159.25 36.81	64.79 17.69	21.70 9.48	8.61 3.19	pmol/ug DNA

*:The units in the table and graphs correspond to the intensity of each analyte divided by the intensity of the corresponding deuterated internal standard. The amount of internal standard added to each sample in the time series and control experiments was identical. The relative intensity values were converted to pmoles by extrapolating from the standard curve for that analyte and then normalized to the number of cells in a given sample as measured by the quantity of DNA in the well.