Lipidomics studies on macrophages - RAW 264.7 cells treated with Kdo2-Lipid A

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Legendgraph icon: Graph of technical replicates(within an expt.)
graph icon: Graph of biological replicates(across expts.)		  (BXY123456): Experiment Sample Barcode
Diacylglycerols| Triacylglycerols
Details/Graph
technical/biological
replicates
 Name KLA
Ctl
 0min		 KLA
Ctl
 30min		 KLA
Ctl
 1hr		 KLA
Ctl
 2hrs		 KLA
Ctl
 4hrs		 KLA
Ctl
 8hrs		 KLA
Ctl
 12hrs		 KLA
Ctl
 24hrs		 Units*
. . . .
mag icon graph icon graph icon(BCE060705)DG(15:0/16:0)_15:0-
0.04		 0.05
0.04		 0.07
0.04		 0.04
0.03		 0.04
0.09		 0.09
0.06		 0.15
0.05		 0.26
0.06		 Ratio Int/ug DNA
mag icon graph icon graph icon(BCE060719)DG(15:0/16:0)_15:0-
0.04		 0.05
0.05		 0.05
0.06		 0.04
0.03		 0.06
0.05		 0.07
0.07		 0.15
0.12		 0.16
0.05		 Ratio Int/ug DNA
mag icon graph icon graph icon(BCE060816)DG(15:0/16:0)_15:0-
0.04		 0.04
0.05		 0.04
0.05		 0.03
0.03		 0.06
0.04		 0.08
0.07		 0.11
0.13		 0.21
0.03		 Ratio Int/ug DNA
*:The units in the table and graphs correspond to the abundance of each [M+NH4]+ ion measured as a neutral loss for a specific fatty acid (+NH3) divided by the signal from the d5-labeled internal standard containing that specific fatty acid, obtained in the same neutral loss scan. The amount of internal standard added to each sample in the time series and control experiments was identical. The ratio of these ion abundances (Ratio Int.) is then normalized to the measured quantity of DNA present in each RAW cell preparation following treatment with and without Kdo2-lipid A for each time point.