Community Forum
The community forum is an informal way to discuss all things lipid and lipidomics related!!
EpiLipidNET is a pan-European network of scientists, clinicians, scientific societies, and enterprises aiming to create a hub of excellence with the focus on integrative EpiLipidomics research. This network is funded by European Cooperation in Science and Technology (COST; CA19105, https://www.cost.eu/actions/CA19105/) and is an open community to all with an interest in (epi)lipids and related technologies. EpiLipidNET community aims to boost multidisciplinary knowledge and technology transfer, and to foster advanced training of young researchers in all aspects related to lipid research.
You can freely view online discussions without login in. You can post your own questions or comments once you have created your own account and are logged in.
We look forward to you joining us on the forum, and contributing to our lipid conversations!
Strategies and standardization for quantitative epilipidomics: analysis of complex oxidized lipids.
On January 10th 2024, 2 pm CET we are planning to have an online discussion about current strategies in analysis of oxidized complex lipids. This online seminar aims to support open discussion on the progress and challenges in current state of the analytical solutions for identification and quantification of oxidized complex lipids (glycerophospholipids and neutral lipids) in biological samples. What can we learn from exciting guidelines in lipidomics and metabolomics and what is very special about oxidized lipids? What is the current state-of-the-art and what do we miss to advance analysis of this exciting class of modified lipids?
We invite you to participate in this open event, share your expertise and ask questions.
In preparation to the seminar, you are welcome to use this Community Forum to post your questions, which will be then discussed during the event. We will also update this thread with the meeting outcomes.
We would like to address the following questions but fill free to add any other questions you think are important!
Lipid extraction strategies to preserve oxidized lipids
Common targeted and untargeted workflows for analysis of complex oxidized lipids
How to quantify analytes with different polarities? What is more important – lipid class, fatty acyl compositions, modification types – when selecting internal standards?
Analytical behaviour: ionization efficiency of lipids with different modification types (hydroxy vs epoxy vs keto), modification type specific in-source fragmentation, differential ionization adducts.
Current status with commercially available internal standards: how many are out there, which ones, why not more (and how many do we need?)
Earlier this year, EpiLipidNET WG2 held an online discussion forum on quantitative epilipidomics.
Thanks to all who took the part in this vivid discussion! Among others topics, availability and the choice of internal standards for oxidized lipids were discussed.
For all of us working with analysis of oxidized/oxygenated lipids, it is indeed a very important question. We would like to hear from you what kind of standards you are using currently in your epilipidomics workflows and what are the open needs in the field.
Lets use the Community Forum platform to discuss our needs and share the available solutions.