LIPID MAPS® Gene/Proteome Database (LMPD)

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LMPD Record

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LMP006825

UniProt Annotations

Entry Information

Gene Name	Vps30p
Protein Entry	BECN1_YEAST
UniProt ID	Q02948
Species	Yeast (S288c)

Comments

Comment type	Description
Domain	The C-terminal domain called the BARA domain is dispensable for the construction of both VPS34 PI3-kinase complexes, but is specifically required for autophagy through the targeting of complex I to the pre-autophagosomal structure
Domain	The C-terminal domain called the BARA domain is dispensable for the construction of both VPS34 PI3-kinase complexes, but is specifically required for autophagy through the targeting of complex I to the pre-autophagosomal structure. {ECO:0000269\|PubMed:22437838}.
Function	Required for cytoplasm to vacuole transport (Cvt), autophagy, nucleophagy, and mitophagy, as a part of the autophagy- specific VPS34 PI3-kinase complex I. This complex is essential to recruit the ATG8-phosphatidylinositol conjugate and the ATG12-ATG5 conjugate to the pre-autophagosomal structure. Also involved in endosome-to-Golgi retrograde transport as part of the VPS34 PI3- kinase complex II. This second complex is required for the endosome-to-Golgi retrieval of PEP1 and KEX2, and the recruitment of VPS5 and VPS7, two components of the retromer complex, to endosomal membranes (probably through the synthesis of a specific pool of phosphatidylinositol 3-phosphate recruiting the retromer to the endosomes). Plays also a role in regulation of filamentous growth. {ECO:0000269\|PubMed:11157979, ECO:0000269\|PubMed:11689437, ECO:0000269\|PubMed:12244127, ECO:0000269\|PubMed:16267277, ECO:0000269\|PubMed:17404498, ECO:0000269\|PubMed:17700056, ECO:0000269\|PubMed:18182384, ECO:0000269\|PubMed:18701704, ECO:0000269\|PubMed:19131141, ECO:0000269\|PubMed:22437838, ECO:0000269\|PubMed:8224160, ECO:0000269\|PubMed:9105038, ECO:0000269\|PubMed:9712845}.
Interaction	P38270:ATG14; NbExp=7; IntAct=EBI-2670, EBI-2699; Q05919:VPS38; NbExp=8; IntAct=EBI-2670, EBI-29972;
Miscellaneous	Present with 2490 molecules/cell in log phase SD medium
Miscellaneous	Present with 2490 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Similarity	Belongs to the beclin family
Similarity	Belongs to the beclin family. {ECO:0000305}.
Subcellular Location	Endosome membrane; Peripheral membrane protein. Vacuole membrane; Peripheral membrane protein. Preautophagosomal structure membrane; Peripheral membrane protein. Note=With the VPS34 PI3-kinase complex I, localizes to the vacuole-isolation membrane contact site (VICS) during isolation membrane (IM) expansion. The IM is a membrane sac generated from the pre-autophagosomal structure that ultimately expands to become a mature autophagosome.
Subunit	Component of the autophagy-specific VPS34 PI3-kinase complex I composed of VPS15, VPS30, VPS34, ATG14 and ATG38; and of the VPS34 PI3-kinase complex II composed of VPS15, VPS30, VPS34 and VPS38
Subunit	Component of the autophagy-specific VPS34 PI3-kinase complex I composed of VPS15, VPS30, VPS34, ATG14 and ATG38; and of the VPS34 PI3-kinase complex II composed of VPS15, VPS30, VPS34 and VPS38. {ECO:0000269\|PubMed:24165940}.