LIPID MAPS® Gene/Proteome Database (LMPD)

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LMP006993

UniProt Annotations

Entry Information
Gene Namemethylsterol monooxygenase
Protein EntryMSMO_YEAST
UniProt IDP53045
SpeciesYeast (S288c)
Comments
Comment typeDescription
Catalytic Activity3-beta-hydroxy-4-beta-methyl-5-alpha-cholest- 7-ene-4-alpha-carbaldehyde + NAD(P)H + O(2) = 3-beta-hydroxy-4- beta-methyl-5-alpha-cholest-7-ene-4-alpha-carboxylate + NAD(P)(+) + H(2)O.
Catalytic Activity4,4-dimethyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)H + O(2) = 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha- cholest-7-en-3-beta-ol + NAD(P)(+) + H(2)O.
Catalytic Activity4-beta-hydroxymethyl-4-alpha-methyl-5-alpha- cholest-7-en-3-beta-ol + NAD(P)H + O(2) = 3-beta-hydroxy-4-beta- methyl-5-alpha-cholest-7-ene-4-alpha-carbaldehyde + NAD(P)(+) + 2 H(2)O.
CofactorName=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000269|PubMed:8663358};
DomainThe histidine box domains may contain the active site and/or be involved in metal ion binding.
FunctionCatalyzes the first step in the removal of the two C-4 methyl groups of 4,4-dimethylzymosterol. {ECO:0000269|PubMed:8663358}.
InteractionP10614:ERG11; NbExp=3; IntAct=EBI-6506, EBI-5127; Q12452:ERG27; NbExp=4; IntAct=EBI-6506, EBI-38132; P40030:ERG28; NbExp=3; IntAct=EBI-6506, EBI-22518; P32353:ERG3; NbExp=3; IntAct=EBI-6506, EBI-6554;
MiscellaneousPresent with 77100 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
PathwaySteroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 3/6.
SimilarityBelongs to the sterol desaturase family. {ECO:0000305}.
Subcellular LocationEndoplasmic reticulum membrane {ECO:0000305|PubMed:12119386, ECO:0000305|PubMed:15716577, ECO:0000305|PubMed:8663358}; Single-pass membrane protein {ECO:0000305|PubMed:12119386, ECO:0000305|PubMed:15716577, ECO:0000305|PubMed:8663358}.
SubunitHeterotetramer of ERG25, ERG26, ERG27 and ERG28. ERG28 acts as a scaffold to tether ERG27 and other 4,4-demethylation- related enzymes, forming a demethylation enzyme complex, in the endoplasmic reticulum. Interacts with ERG27 and ERG28. {ECO:0000269|PubMed:12119386, ECO:0000269|PubMed:15995173}.