LIPID MAPS® Gene/Proteome Database (LMPD)

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LMPD Record

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LMP007189

UniProt Annotations

Entry Information
Gene Namebranched-chain-2-oxoacid decarboxylase THI3
Protein EntryTHI3_YEAST
UniProt IDQ07471
SpeciesYeast (S288c)
Comments
Comment typeDescription
BiotechnologyFusel oils are important flavor and aroma compounds in yeast-fermented products contributing to the quality of beverages and food. In low concentration they are generally desirable, whereas high concentrations may spoil the product. By adjusting growth conditions and substrate their production is sought to be influenced.
Catalytic ActivityA 2-oxo acid = an aldehyde + CO(2).
CofactorName=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250}; Note=Binds 1 Mg(2+) per subunit. {ECO:0000250};
CofactorName=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence= ; Note=Binds 1 Mg(2+) per subunit. ;
CofactorName=thiamine diphosphate; Xref=ChEBI:CHEBI:58937; Evidence={ECO:0000250}; Note=Binds 1 thiamine pyrophosphate per subunit. {ECO:0000250};
CofactorName=thiamine diphosphate; Xref=ChEBI:CHEBI:58937; Evidence= ; Note=Binds 1 thiamine pyrophosphate per subunit. ;
FunctionOne of five 2-oxo acid decarboxylases (PDC1, PDC5, PDC6, ARO10, and THI3) involved in amino acid catabolism. The enzyme catalyzes the decarboxylation of amino acids, which, in a first step, have been transaminated to the corresponding 2-oxo acids (alpha-keto-acids). In a third step, the resulting aldehydes are reduced to alcohols, collectively referred to as fusel oils or alcohols. Its preferred substrates are the transaminated amino acids leucine and isoleucine, whereas valine, aromatic amino acids, and pyruvate are no substrates. In analogy to the pyruvate decarboxylases the enzyme may in a side-reaction catalyze condensation (or carboligation) reactions leading to the formation of 2-hydroxy ketone, collectively called acyloins. The enzyme is also positively regulating the thiamine metabolism by a molecular mechanism that may involve thiamine concentration sensing and signal transmission. {ECO:0000269|PubMed:10753893, ECO:0000269|PubMed:12499363, ECO:0000269|PubMed:12902239, ECO:0000269|PubMed:1624458, ECO:0000269|PubMed:9341119, ECO:0000269|PubMed:9748245}.
InteractionP32896:PDC2; NbExp=3; IntAct=EBI-19209, EBI-13004;
MiscellaneousPresent with 2140 molecules/cell in log phase SD medium
MiscellaneousPresent with 2140 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
PathwayAmino-acid degradation; Ehrlich pathway.
SimilarityBelongs to the TPP enzyme family
SimilarityBelongs to the TPP enzyme family. {ECO:0000305}.
Subcellular LocationNucleus .
Subcellular LocationNucleus {ECO:0000269|PubMed:14562095}.