LIPID MAPS® Gene/Proteome Database (LMPD)

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LMPD Record

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LMP007586

UniProt Annotations

Entry Information
Gene NameUDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase
Protein EntryWECA_ECOLI
UniProt IDP0AC78
SpeciesE. coli
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=5.6 uM for UDP-GlcNAc {ECO:0000269|PubMed:9134438};
Catalytic ActivityUDP-N-acetyl-alpha-D-glucosamine + ditrans,octacis-undecaprenyl phosphate = UMP + N-acetyl-alpha-D- glucosaminyl-diphospho-ditrans,octacis-undecaprenol.
CofactorName=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:17237164};
CofactorName=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000269|PubMed:17237164};
Enzyme RegulationInhibited by tunicamycin.
FunctionCatalyzes the transfer of the GlcNAc-1-phosphate moiety from UDP-GlcNAc onto the carrier lipid undecaprenyl phosphate (C55-P), yielding GlcNAc-pyrophosphoryl-undecaprenyl (GlcNAc-PP- C55). It is the first lipid-linked intermediate involved in enterobacterial common antigen (ECA) synthesis and an acceptor for the addition of subsequent sugars to complete the biosynthesis of O-antigen. The apparent affinity of WecA for the polyisoprenyl phosphate substrates increases with the polyisoprenyl chain length. WecA is unable to utilize dolichyl phosphate (Dol-P). {ECO:0000269|PubMed:11700352, ECO:0000269|PubMed:1730666}.
PathwayBacterial outer membrane biogenesis; LPS O-antigen biosynthesis.
Sequence CautionSequence=AAA24526.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=AAB20842.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};
SimilarityBelongs to the glycosyltransferase 4 family. WecA subfamily. {ECO:0000305}.
Subcellular LocationCell inner membrane; Multi-pass membrane protein.