LIPID MAPS® Gene/Proteome Database (LMPD)

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LMPD Record

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LMP007719

UniProt Annotations

Entry Information
Gene Namecolanic biosynthesis UDP-glucose lipid carrier transferase
Protein EntryWCAJ_ECOLI
UniProt IDP71241
SpeciesE. coli
Comments
Comment typeDescription
Catalytic ActivityUDP-glucose + ditrans,octacis-undecaprenyl phosphate = UMP + alpha-D-glucopyranosyl-diphospho- ditrans,octacis-undecaprenol. {ECO:0000269|PubMed:22408159}.
Disruption PhenotypeCells lacking this gene show a non-mucoid phenotype. {ECO:0000269|PubMed:22408159}.
FunctionIs the initiating enzyme for colanic acid (CA) synthesis. Catalyzes the transfer of the glucose-1-phosphate moiety from UDP-Glc onto the carrier lipid undecaprenyl phosphate (C55-P), forming a phosphoanhydride bond yielding to glucosyl- pyrophosphoryl-undecaprenol (Glc-PP-C55). Also possesses a weak galactose-1-P transferase activity. {ECO:0000269|PubMed:22408159}.
MiscellaneousComplements holdfast synthesis in the C.crescentus mutant lacking hfsE, pssY and pssZ genes. Can also partially complement the O-antigen defect in the S.typhimurium wbaP deletion strain (PubMed:22408159). {ECO:0000305|PubMed:22408159}.
PathwayExopolysaccharide biosynthesis; colanic acid biosynthesis. {ECO:0000269|PubMed:22408159}.
SimilarityBelongs to the bacterial sugar transferase family. {ECO:0000305}.
Subcellular LocationCell inner membrane {ECO:0000269|PubMed:22408159}; Multi-pass membrane protein {ECO:0000269|PubMed:22408159}.