LIPID MAPS® Gene/Proteome Database (LMPD)

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LMPD Record

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LMP010596

UniProt Annotations

Entry Information
Gene Namemalonate--CoA ligase
Protein EntryAAE13_ARATH
UniProt IDQ8H151
SpeciesArabidopsis
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=530 uM for malonate (at pH 7.5) {ECO:0000269|PubMed:21642549}; Vmax=24 umol/min/mg enzyme toward malonate (at pH 7.5) {ECO:0000269|PubMed:21642549}; pH dependence: Optimum pH is 6.5-8.0. {ECO:0000269|PubMed:21642549};
Catalytic ActivityATP + malonate + CoA = AMP + diphosphate + malonyl-CoA. {ECO:0000269|PubMed:21642549}.
Disruption PhenotypeStrong defects in growth and development and most of the plants die before flowering. Viable plants have a greatly reduced seed set with a low proportion of viable seed and accumulate malonate and succinate. {ECO:0000269|PubMed:21642549}.
FunctionMalonate--CoA ligase that catalyzes the formation of malonyl-CoA directly from malonate and CoA. May be required for the detoxification of malonate. {ECO:0000269|PubMed:21642549}.
InductionBy abscisic acid (ABA) and osmotic stress. {ECO:0000269|PubMed:21642549}.
MiscellaneousPlants overexpressing AAE13 show increased sensitivity to exogenous malonate.
Sequence CautionSequence=BAB02683.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
SimilarityBelongs to the ATP-dependent AMP-binding enzyme family. {ECO:0000305}.
Subcellular LocationCytoplasm {ECO:0000269|PubMed:21642549}. Nucleus {ECO:0000269|PubMed:21642549}.
Tissue SpecificityExpressed in flowers. {ECO:0000269|PubMed:12805634, ECO:0000269|PubMed:21642549}.