LIPID MAPS® Gene/Proteome Database (LMPD)

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LMPD Record

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LMP010619

UniProt Annotations

Entry Information
Gene Namesterol-4alpha-methyl oxidase 1-1
Protein EntrySMO11_ARATH
UniProt IDQ8L7W5
SpeciesArabidopsis
Comments
Comment typeDescription
Catalytic Activity3-beta-hydroxy-4-beta-methyl-5-alpha-cholest- 7-ene-4-alpha-carbaldehyde + NAD(P)H + O(2) = 3-beta-hydroxy-4- beta-methyl-5-alpha-cholest-7-ene-4-alpha-carboxylate + NAD(P)(+) + H(2)O. {ECO:0000269|PubMed:14653780}.
Catalytic Activity4,4-dimethyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)H + O(2) = 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha- cholest-7-en-3-beta-ol + NAD(P)(+) + H(2)O. {ECO:0000269|PubMed:14653780}.
Catalytic Activity4-beta-hydroxymethyl-4-alpha-methyl-5-alpha- cholest-7-en-3-beta-ol + NAD(P)H + O(2) = 3-beta-hydroxy-4-beta- methyl-5-alpha-cholest-7-ene-4-alpha-carbaldehyde + NAD(P)(+) + 2 H(2)O. {ECO:0000269|PubMed:14653780}.
CofactorName=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000250};
DomainThe histidine box domains may contain the active site and/or be involved in metal ion binding.
FunctionNon-heme iron oxygenase involved in sterols biosynthesis. 4,4-dimethyl-9-beta,19-cyclopropylsterols such as 24-methylenecycloartanol are the preferred substrates. {ECO:0000269|PubMed:14653780}.
MiscellaneousRequires a membrane-bound cytochrome b5 as an obligatory electron carrier from NAD(P)H to SMO. {ECO:0000250}.
Sequence CautionSequence=CAB40952.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; Sequence=CAB78254.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
SimilarityBelongs to the sterol desaturase family. {ECO:0000305}.
Subcellular LocationEndoplasmic reticulum membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.