LIPID MAPS® Gene/Proteome Database (LMPD)

Introduction | Overview of Interfaces | Browse | Search | Download

LMPD Record

New search | Record Overview | Ontology/Pathway Information | Domain Information | UniProt Annotations | Related Proteins
LMP010622

UniProt Annotations

Entry Information
Gene Namesterol 4-alpha-methyl-oxidase 2-2
Protein EntrySMO21_ARATH
UniProt IDQ9ZW22
SpeciesArabidopsis
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=5; Name=1; IsoId=Q9ZW22-1; Sequence=Displayed; Name=2; IsoId=Q9ZW22-2; Sequence=VSP_041864; Note=No experimental confirmation available.; Name=3; IsoId=Q9ZW22-3; Sequence=VSP_041862, VSP_041864; Note=No experimental confirmation available.; Name=4; IsoId=Q9ZW22-4; Sequence=VSP_041860, VSP_041864; Note=Derived from EST data. No experimental confirmation available.; Name=5; IsoId=Q9ZW22-5; Sequence=VSP_041861, VSP_041863, VSP_041864; Note=Derived from EST data.;
Catalytic Activity3-beta-hydroxy-4-beta-methyl-5-alpha-cholest- 7-ene-4-alpha-carbaldehyde + NAD(P)H + O(2) = 3-beta-hydroxy-4- beta-methyl-5-alpha-cholest-7-ene-4-alpha-carboxylate + NAD(P)(+) + H(2)O.
Catalytic Activity4,4-dimethyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)H + O(2) = 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha- cholest-7-en-3-beta-ol + NAD(P)(+) + H(2)O.
Catalytic Activity4-beta-hydroxymethyl-4-alpha-methyl-5-alpha- cholest-7-en-3-beta-ol + NAD(P)H + O(2) = 3-beta-hydroxy-4-beta- methyl-5-alpha-cholest-7-ene-4-alpha-carbaldehyde + NAD(P)(+) + 2 H(2)O.
CofactorName=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000250};
DomainThe histidine box domains may contain the active site and/or be involved in metal ion binding.
FunctionNon-heme iron oxygenase involved in sterols biosynthesis. 24-ethylidenelophenol and 24-ethyllophenol are the preferred substrates. {ECO:0000269|PubMed:11707264}.
MiscellaneousRequires a membrane-bound cytochrome b5 as an obligatory electron carrier from NAD(P)H to SMO.
SimilarityBelongs to the sterol desaturase family. {ECO:0000305}.
Subcellular LocationEndoplasmic reticulum membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.