LIPID MAPS® Gene/Proteome Database (LMPD)

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LMPD Record

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LMP010646

UniProt Annotations

Entry Information
Gene NameNADP-malic enzyme 4
Protein EntryMAOP4_ARATH
UniProt IDQ9CA83
SpeciesArabidopsis
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=10.2 uM for NADP (at pH 7.5) {ECO:0000269|PubMed:16113210}; KM=0.23 mM for malate (at pH 7.5) {ECO:0000269|PubMed:16113210}; Note=kcat is 151.3 sec(-1) with NADP as substrate (at pH 7.5).;
Catalytic ActivityOxaloacetate = pyruvate + CO(2). {ECO:0000269|PubMed:16113210}.
Catalytic Activity(S)-malate + NADP(+) = pyruvate + CO(2) + NADPH. {ECO:0000269|PubMed:16113210}.
CofactorName=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250}; Note=Divalent metal cations. Prefers magnesium or manganese. {ECO:0000250};
Developmental StageDuring embryogenesis, present in the endosperm and the embryo at all developmental stages, including the seed attachment point and the integuments. Also detected in siliques. During germination, first confined to the radicle to later approaches the meristematic region. Finally present in the whole root. Expressed in cotyledons and primary leaves. In opened flowers, present in sepals, stigma, filaments, and pollen. {ECO:0000269|PubMed:16113210}.
FunctionThe chloroplastic ME isoform decarboxylates malate shuttled from neighboring mesophyll cells. The CO(2) released is then refixed by ribulose-bisphosphate carboxylase. This pathway eliminates the photorespiratory loss of CO(2) that occurs in most plants (By similarity). {ECO:0000250}.
PathwayPhotosynthesis; C3 acid pathway.
Sequence CautionSequence=AAF68116.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; Sequence=BAD94826.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};
SimilarityBelongs to the malic enzymes family. {ECO:0000305}.
Subcellular LocationPlastid, chloroplast {ECO:0000269|PubMed:16113210, ECO:0000269|PubMed:21841088}.
SubunitHomodimer and homotetramer. {ECO:0000269|PubMed:16113210}.
Tissue SpecificityExpressed in leaves, stems, flowers and roots, mainly in vascular system. In roots, present in the stele, including the vascular tissue and the pericycle, mainly at emerging lateral roots and at root tips. {ECO:0000269|PubMed:16113210}.