LIPID MAPS® Gene/Proteome Database (LMPD)

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LMPD Record

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LMP010876

UniProt Annotations

Entry Information
Gene Namephospholipase D gamma 1
Protein EntryPLDG1_ARATH
UniProt IDQ9T053
SpeciesArabidopsis
Comments
Comment typeDescription
Catalytic ActivityA phosphatidylcholine + H(2)O = choline + a phosphatidate.
CautionIt is uncertain whether Met-1 or Met-11 is the initiator. {ECO:0000305}.
CofactorName=Ca(2+); Xref=ChEBI:CHEBI:29108; Note=Ca(2+). Requires micromolar level (PIP2-dependent).;
DomainC2 domain is a calcium-binding fold, and the binding promotes the protein association with membranes. In PLD gamma, all the calcium-coordinating acidic amino acids are conserved.
Enzyme RegulationInhibited by neomycin.
FunctionHydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond. Plays an important role in various cellular processes, including phytohormone action, vesicular trafficking, secretion, cytoskeletal arrangement, meiosis, tumor promotion, pathogenesis, membrane deterioration and senescence. Can use phosphatidylserine but prefers ethanolamine-containing lipids as substrates.
InductionActivated by wounding, heavy metal, methyl salicylate, osmotic and salt stresses.
Sequence CautionSequence=AAB87672.1; Type=Frameshift; Positions=60, 79, 260, 300, 465, 758; Evidence={ECO:0000305};
SimilarityBelongs to the phospholipase D family. C2-PLD subfamily. {ECO:0000305}.
SimilarityContains 1 C2 domain. {ECO:0000255|PROSITE- ProRule:PRU00041}.
SimilarityContains 2 PLD phosphodiesterase domains. {ECO:0000255|PROSITE-ProRule:PRU00153}.
Subcellular LocationCytoplasm {ECO:0000269|PubMed:10198096}. Membrane {ECO:0000269|PubMed:10198096}; Peripheral membrane protein {ECO:0000269|PubMed:10198096}. Note=Found mainly associated with intracellular membranes but also with mitochondrial membranes, nuclei and clathrin-coated vesicles. Not found in chloroplast.
Tissue SpecificityHighly expressed in roots and flowers, moderately in stems, leaves and seedlings and low in siliques. Not detected in seeds.