LIPID MAPS® Gene/Proteome Database (LMPD)

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LMPD Record

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LMP012950

UniProt Annotations

Entry Information
Gene Namealpha 1,3-galactosyltransferase 2
Protein EntryA3LT2_RAT
UniProt IDA0A4Z3
SpeciesRat
Comments
Comment typeDescription
Catalytic ActivityUDP-alpha-D-galactose + beta-D-galactosyl- (1->4)-beta-N-acetyl-D-glucosaminyl-R = UDP + alpha-D-galactosyl- (1->3)-beta-D-galactosyl-(1->4)-beta-N-acetylglucosaminyl-R.
CofactorName=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence= ;
DomainThe conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis.
FunctionSynthesizes the galactose-alpha(1,3)-galactose group on the glycosphingolipid isoglobotrihexosylceramide or isogloboside 3 (iGb3) by catalyzing the transfer of galactose from UDP-Galactose to its acceptor molecule Gal-beta-1,4-Glc-ceramide. Can also catalyze the addition of galactose to iGb3 itself to form polygalactose structures. Synthesis of iGb3 is the initial step in the formation of the isoglobo-series glycolipid pathway and is the precursor to isogloboside 4 (iGb4) and isoForssman glycolipids. Can glycosylate only lipids and not proteins and is solely responsible for initiating the synthesis of isoglobo-series glycosphingolipids. {ECO:0000269|PubMed:10854427, ECO:0000269|PubMed:12626403, ECO:0000269|PubMed:17206613}.
MiscellaneousThis gene is not expressed in humans, and even if it were to be expressed, this enzyme appears to be inactive because of multiple mutations including an amino-acid substitution Leu-187 to Pro and Tyr-252 to Asn.
SimilarityBelongs to the glycosyltransferase 6 family
Subcellular LocationGolgi apparatus, Golgi stack membrane ; Single-pass type II membrane protein {ECO:0000269|PubMed:17206613, ECO:0000269|PubMed:18630988}. Note=Also found in numerous large vesicles throughout the cytoplasm of the soma.
Tissue SpecificityDorsal root ganglia neurons (at protein level). High levels in spleen, thymus and skeletal muscle, intermediate levels in lung, uterus, pituitary and heart, low levels in brain, and undetected in adrenal gland and liver. {ECO:0000269|PubMed:10854427, ECO:0000269|PubMed:12626403, ECO:0000269|PubMed:17206613}.