LMPD Database

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LMP000691

UniProt Annotations

Entry Information
Gene Nameacyl-CoA thioesterase 1
Protein EntryACOT1_MOUSE
UniProt IDO55137
SpeciesMouse
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=2.6 uM for palmitoyl-CoA {ECO:0000269|PubMed:11694534}; Vmax=1.2 umol/min/mg enzyme with palmitoyl-CoA as substrate {ECO:0000269|PubMed:11694534};
Catalytic ActivityPalmitoyl-CoA + H(2)O = CoA + palmitate.
FunctionAcyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. True acyl-CoA thioesterase being highly specific for saturated C12-C20 acyl-CoAs, with only a very low activity with decanoyl-CoA as substrate. Most active on myristoyl- and palmitoyl-CoA. Introduction of one or two double bonds decreases the activity to about half. No detectable activity with bile acid CoAs such as choloyl-CoA and chenodeoxycholoyl-CoA. {ECO:0000269|PubMed:10567408}.
InductionIn the liver, by peroxisome proliferator (Clofibrate) treatment, via the peroxisome proliferator-activated receptors (PPARs) or fasting for 24 hours. {ECO:0000269|PubMed:11330065}.
SimilarityBelongs to the C/M/P thioester hydrolase family. {ECO:0000305}.
Subcellular LocationCytoplasm.
SubunitMonomer.
Tissue SpecificityExpressed in heart, kidney, brown adipose tissue, white adipose tissue, adrenal gland and muscle. {ECO:0000269|PubMed:10567408, ECO:0000269|PubMed:11330065}.