LMPD Database

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LMP001611

UniProt Annotations

Entry Information
Gene NameATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1
Protein EntryP70704_MOUSE
UniProt IDP70704
SpeciesMouse
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=3; Name=1; IsoId=P70704-1; Sequence=Displayed; Name=2; IsoId=P70704-2; Sequence=VSP_055306, VSP_055307; Name=3; IsoId=P70704-3; Sequence=VSP_055307;
Catalytic ActivityATP + H(2)O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).
CautionInitial characterization studies with purified Atp8a1 enzyme demonstrated similar but distinct properties compared to the plasma membrane aminophospholipid flippase; however, the flippase complex accessory beta subunit was not included in the assays. {ECO:0000305|PubMed:16618126}.
Enzyme RegulationATPase activity is stimulated by phosphatidylserine (PS) and minimally by phosphatidylethanolamine (PE). {ECO:0000269|PubMed:16618126}.
FunctionCatalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. In vitro, its ATPase activity is selectively and stereospecifically stimulated by phosphatidylserine (PS). The flippase complex ATP8A1:TMEM30A seems to play a role in regulation of cell migration probably involving flippase-mediated translocation of phosphatidylethanolamine (PE) at the plasma membrane. Acts as aminophospholipid translocase at the plasma membrane in neuronal cells; the activity is associated with hippocampus-dependent learning. {ECO:0000269|PubMed:16618126, ECO:0000269|PubMed:20224745, ECO:0000269|PubMed:22007859, ECO:0000269|PubMed:23269685}.
SimilarityBelongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily. {ECO:0000305}.
Subcellular LocationCytoplasmic vesicle, secretory vesicle, chromaffin granule membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. Cytoplasmic granule {ECO:0000250}. Cell membrane {ECO:0000250}. Endoplasmic reticulum {ECO:0000250}. Golgi apparatus {ECO:0000250}. Note=Exit from the endoplasmic reticulum requires the presence of TMEM30A, but not TMEM30B. In the presence of TMEM30A, predominantly located in cytoplasmic punctate structures (By similarity). Localizes to plasma membranes of red blood cells. {ECO:0000250, ECO:0000269|PubMed:16643453}.
SubunitComponent of a P4-ATPase flippase complex which consists of a catalytic alpha subunit and an accessory beta subunit. Interacts with TMEM30A to form a flippase complex. {ECO:0000269|PubMed:23269685}.
Tissue SpecificityFound in most tissues except liver and testis. Most abundant in brain and lung. Also detected in fetal tissues. Isoform 1 is expressed in brain. Isoform 2 and isoform 3 are expressed in reticulocytes. {ECO:0000269|PubMed:16643453}.