LMPD Database

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LMP004629

UniProt Annotations

Entry Information
Gene Nameplatelet-activating factor acetylhydrolase, isoform 1b, subunit 1
Protein EntryLIS1_MOUSE
UniProt IDP63005
SpeciesMouse
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=2; Name=1; IsoId=P63005-1, P43035-1; Sequence=Displayed; Name=2; IsoId=P63005-2, P43035-2; Sequence=VSP_006778;
Developmental StageEmbryonic expression begins prior to the blastocyst stage, when maternally expressed protein is depleted. By E10.5, expression is abundant in the developing central and peripheral nervous systems. Major sites of expression include the neuroepithelium of the fore-, mid-, and hindbrain, the spinal cord, the dorsal root and the cranial ganglia. By E13.5, highly expressed in neuroblasts as well as postmitotic neurons of the cortical plate. After completion of neuronal migration expression remains high in the cortex. Also expressed in the testis from P8. {ECO:0000269|PubMed:11001923, ECO:0000269|PubMed:11056530, ECO:0000269|PubMed:11163259, ECO:0000269|PubMed:11231056, ECO:0000269|PubMed:11344260, ECO:0000269|PubMed:12950100, ECO:0000269|PubMed:15147871, ECO:0000269|PubMed:15473966}.
DomainDimerization mediated by the LisH domain may be required to activate dynein. {ECO:0000255|HAMAP-Rule:MF_03141, ECO:0000269|PubMed:15274919, ECO:0000269|PubMed:16258276}.
FunctionPositively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein- mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Neuronal migration involves a process called nucleokinesis, whereby migrating cells extend an anterior process into which the nucleus subsequently translocates. During nucleokinesis dynein at the nuclear surface may translocate the nucleus towards the centrosome by exerting force on centrosomal microtubules. Also required for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors. May also play a role in other forms of cell locomotion including the migration of fibroblasts during wound healing. Non-catalytic subunit of an acetylhydrolase complex which inactivates platelet-activating factor (PAF) by removing the acetyl group at the SN-2 position. {ECO:0000255|HAMAP-Rule:MF_03141, ECO:0000269|PubMed:11056530, ECO:0000269|PubMed:11344260, ECO:0000269|PubMed:12796778, ECO:0000269|PubMed:12911752, ECO:0000269|PubMed:14507966, ECO:0000269|PubMed:14578885, ECO:0000269|PubMed:14691133, ECO:0000269|PubMed:15173193, ECO:0000269|PubMed:15473966, ECO:0000269|PubMed:16107726, ECO:0000269|PubMed:16203747, ECO:0000269|PubMed:16369480, ECO:0000269|PubMed:16481446}.
InteractionQ9ERR1:Ndel1; NbExp=4; IntAct=EBI-917499, EBI-646668; Q61205:Pafah1b3; NbExp=2; IntAct=EBI-917499, EBI-1007637;
SimilarityBelongs to the WD repeat LIS1/nudF family. {ECO:0000255|HAMAP-Rule:MF_03141}.
SimilarityContains 1 LisH domain. {ECO:0000255|HAMAP- Rule:MF_03141}.
SimilarityContains 7 WD repeats. {ECO:0000255|HAMAP- Rule:MF_03141}.
Subcellular LocationCytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Nucleus membrane {ECO:0000255|HAMAP-Rule:MF_03141}. Note=May localize to the nuclear membrane (By similarity). Localizes to the plus end of microtubules and to the centrosome. Redistributes to axons during neuronal development. Also localizes to the microtubules of the manchette in elongating spermatids and to the meiotic spindle in spermatocytes. {ECO:0000250}.
SubunitInteracts with DISC1, and this interaction is enhanced by NDEL1 (By similarity). Component of cytosolic PAF-AH IB, which is composed of PAFAH1B1 (alpha), PAFAH1B2 (beta) and PAFAH1B3 (gamma) subunits. Trimer formation is not essential for the catalytic activity of the enzyme which is contributed solely by the PAFAH1B2 (beta) and PAFAH1B3 (gamma) subunits. Can self-associate. Interacts with DCX, dynein, dynactin, IQGAP1, KATNB1, NDE1, NDEL1, NUDC, and RSN. Interacts with DAB1 when DAB1 is phosphorylated in response to RELN/reelin signaling. Interacts with ASUN. {ECO:0000250, ECO:0000269|PubMed:11001923, ECO:0000269|PubMed:11056530, ECO:0000269|PubMed:11163258, ECO:0000269|PubMed:11163259, ECO:0000269|PubMed:11163260, ECO:0000269|PubMed:11231056, ECO:0000269|PubMed:11344260, ECO:0000269|PubMed:11940666, ECO:0000269|PubMed:12885786, ECO:0000269|PubMed:14578885, ECO:0000269|PubMed:15173193, ECO:0000269|PubMed:15473966, ECO:0000269|PubMed:15473967, ECO:0000269|PubMed:15572112, ECO:0000269|PubMed:16203747, ECO:0000269|PubMed:16291865, ECO:0000269|PubMed:16369480, ECO:0000269|PubMed:16481446, ECO:0000269|PubMed:23097494}.
Tissue SpecificityHighly expressed in brain, particularly the hippocampus and the olfactory bulb. Also highly expressed in testis, including all seminiferous tubule cell types, all types of spermatogenic and Sertoli cells, and meiotically dividing and elongating spermatids. Expressed at lower levels in heart, kidney, large intestine, liver, lung, ovary, small intestine and spleen. {ECO:0000269|PubMed:11163259, ECO:0000269|PubMed:11163260, ECO:0000269|PubMed:12551946}.