LMPD Database

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LMP006815

UniProt Annotations

Entry Information
Gene Namediacylglycerol cholinephosphotransferase
Protein EntryCPT1_YEAST
UniProt IDP17898
SpeciesYeast (S288c)
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=100 uM for CDP-choline {ECO:0000269|PubMed:1847919}; KM=137 uM for CDP-dimethylethanolamine {ECO:0000269|PubMed:1847919}; Vmax=0.2 nmol/min/mg enzyme for CDP-choline {ECO:0000269|PubMed:1847919}; Vmax=0.07 nmol/min/mg enzyme for CDP-dimethylethanolamine {ECO:0000269|PubMed:1847919};
Catalytic ActivityCDP-choline + 1,2-diacyl-sn-glycerol = CMP + a phosphatidylcholine. {ECO:0000269|PubMed:15225629, ECO:0000269|PubMed:1847919}.
CofactorName=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:1847919};
Enzyme RegulationRequires a divalent cation activator, and is inhibited by CMP. Activated by phospholipids, especially phosphatidylcholine. {ECO:0000269|PubMed:1847919}.
FunctionCatalyzes the final step in the CDP-choline route leading to phosphatidylcholin (PC). Preferentially uses CDP- monomethylethanolamine as aminoalcohol substrate. Shows highest activity toward di- and mono-unsaturated diacylglycerol species as lipid substrates. The CDP-choline pathway only contributes to net PC synthesis if exogenous choline is present. In its absence, this pathway recycles choline from PC turnover and may contribute to maintaining the proper PC species composition. {ECO:0000269|PubMed:15225629, ECO:0000269|PubMed:1847919, ECO:0000269|PubMed:3029130, ECO:0000269|PubMed:7961445, ECO:0000269|PubMed:7961735}.
InductionRepressed by inositol. {ECO:0000269|PubMed:7961831}.
MiscellaneousPresent with 981 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
PathwayPhospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 2/2.
Sequence CautionSequence=AAA63571.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=CAA86895.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=CAA96012.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
SimilarityBelongs to the CDP-alcohol phosphatidyltransferase class-I family. {ECO:0000305}.
Subcellular LocationMicrosome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Mitochondrion outer membrane; Multi-pass membrane protein.