LMPD Database

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LMP006817

UniProt Annotations

Entry Information
Gene NameTam41p
Protein EntryTAM41_YEAST
UniProt IDP53230
SpeciesYeast (S288c)
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=0.76 mM for CTP {ECO:0000269|PubMed:23623749}; KM=0.067 mM for nitrobenzoxadiazole-phosphatidate {ECO:0000269|PubMed:23623749}; Vmax=68 nmol/min/mg enzyme {ECO:0000269|PubMed:23623749}; pH dependence: Optimum pH is 7-9. {ECO:0000269|PubMed:23623749};
Catalytic ActivityCTP + phosphatidate = diphosphate + CDP- diacylglycerol. {ECO:0000269|PubMed:23623749}.
CautionWas initially identified as protein involved in the translocation of transit peptide-containing proteins across the mitochondrial inner membrane by its role in maintaining the integrity and stability of the inner membrane translocase TIM23 complex (PubMed:16943180, PubMed:16790493). It has later been shown that this phenotype can be attributed to the pleiotropic effects of mitochondria lacking cardiolipin (PubMed:19114592). {ECO:0000305|PubMed:16790493, ECO:0000305|PubMed:16943180, ECO:0000305|PubMed:19114592}.
CofactorName=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:23623749}; Name=Co(2+); Xref=ChEBI:CHEBI:48828; Evidence={ECO:0000269|PubMed:23623749}; Name=Cu(2+); Xref=ChEBI:CHEBI:29036; Evidence={ECO:0000269|PubMed:23623749}; Note=Magnesium. Also active with cobalt or copper. {ECO:0000269|PubMed:23623749};
Disruption PhenotypeDeficient in cardiolipin. Blocked in the import of presequence-containing proteins as well as of non- cleavable carrier proteins into mitochondria. {ECO:0000269|PubMed:19114592}.
FunctionCatalyzes the formation of CDP-diacylglycerol (CDP-DAG) from phosphatidic acid (PA) in the mitochondrial inner membrane. Required for the biosynthesis of the dimeric phospholipid cardiolipin, which stabilizes supercomplexes of the mitochondrial respiratory chain in the mitochondrial inner membrane. {ECO:0000269|PubMed:16790493, ECO:0000269|PubMed:16943180, ECO:0000269|PubMed:19114592, ECO:0000269|PubMed:23623749}.
InteractionP19882:HSP60; NbExp=1; IntAct=EBI-23156, EBI-8586;
MiscellaneousPresent with 195 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
PathwayPhospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3. {ECO:0000269|PubMed:19114592, ECO:0000269|PubMed:20485265}.
SimilarityBelongs to the TAM41 family. {ECO:0000305}.
Subcellular LocationMitochondrion inner membrane {ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:16790493, ECO:0000269|PubMed:16943180, ECO:0000269|PubMed:23623749}; Peripheral membrane protein {ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:16790493, ECO:0000269|PubMed:16943180, ECO:0000269|PubMed:23623749}; Matrix side {ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:16790493, ECO:0000269|PubMed:16943180, ECO:0000269|PubMed:23623749}.